PRODUCTION OF CLOSTRIDIUM BOTULINUM TYPE E ANTITOXIN

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MINAEI M.E.; SAADATI MOJTABA

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF MILITARY MEDICINE Year:2010 | Volume:12 | Issue:1 (43) Page(s): 51-56

Download Full-Text

Persian Verion

View:

3,205

Download:

Cites:

Information Journal Paper

Title

PRODUCTION OF CLOSTRIDIUM BOTULINUM TYPE E ANTITOXIN

Author(s)

MINAEI M.E. | SAADATI MOJTABA | Issue Writer Certificate

Keywords

CLOSTRIDIUM BOTULINUMQ3

BOTULINUM TOXIN TYPE EQ3

ANTITOXINQ3

Abstract

Aims: The Botulinum toxin is known as the most potent toxin. In addition to the therapeutic applications in medicine (Botox), this toxin is also a threat in biological warfare and bioterrorism. Preparing the antibody against the CLOSTRIDIUM BOTULINUM toxin type A and G, despite the application in identifying strains of CLOSTRIDIUM BOTULINUM toxigenic plays an important role in the treatment of poisoning caused by this toxin. The purpose of this study was to produce CLOSTRIDIUM BOTULINUM type E ANTITOXIN.Methods: For immunization of lab animals (chicken, mouse and rabbit),at first, the toxoid was injected with Freund's complete adjuvant either subcutaneously or intramuscularly and in the first to third booster injection ,it was injected with Freund's incomplete adjuvant. At the end or intervals of injections, blood sampling was done and the produced antibody’s titer was measured using the enzyme-linked immunosorbent assay or ELISA method.Results: After each booster injection, a considerable increase in serum antibody titer of lab animals was seen. The titer of CLOSTRIDIUM BOTULINUM type E toxin antibodies in the serum of chicken, rabbit and mouse increased to more than 1:3200. After purification of antibody, less than 0.156 microgram of the purified mouse antibody and less than 0.312 microgram of rabbit purified antibody were able to detect the toxin. Conclusion: After the injection of toxoid, antibody increase in the body of lab animal. Production of specific ANTITOXIN for detection or diagnosis of botulism, based on reaction between antigen and antibody and also for treatment of poisoning is necessary.

Cites

No record.

References

Cite

APA: Copy

MINAEI, M.E., & SAADATI, MOJTABA. (2010). PRODUCTION OF CLOSTRIDIUM BOTULINUM TYPE E ANTITOXIN. JOURNAL OF MILITARY MEDICINE, 12(1 (43)), 51-56. SID. https://sid.ir/paper/61906/en

Vancouver: Copy

MINAEI M.E., SAADATI MOJTABA. PRODUCTION OF CLOSTRIDIUM BOTULINUM TYPE E ANTITOXIN. JOURNAL OF MILITARY MEDICINE[Internet]. 2010;12(1 (43)):51-56. Available from: https://sid.ir/paper/61906/en

IEEE: Copy

M.E. MINAEI, and MOJTABA SAADATI, “PRODUCTION OF CLOSTRIDIUM BOTULINUM TYPE E ANTITOXIN,” JOURNAL OF MILITARY MEDICINE, vol. 12, no. 1 (43), pp. 51–56, 2010, [Online]. Available: https://sid.ir/paper/61906/en

Related Journal Papers