PROTECTIVE EFFECTS OF MORPHINE LOW CONCENTRATION ON DEXAMETHASONE-INDUCED CELL DEATH IN DIFFERENTIATED PC12 CELLS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

NAJMI NEJAD S.; ZHALEH H.; AZADBAKHT M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS) Year:2013 | Volume:15 | Issue:5 Page(s): 7-16

Download Full-Text

Persian Verion

View:

924

Download:

Cites:

Information Journal Paper

Title

PROTECTIVE EFFECTS OF MORPHINE LOW CONCENTRATION ON DEXAMETHASONE-INDUCED CELL DEATH IN DIFFERENTIATED PC12 CELLS

Author(s)

NAJMI NEJAD S. | ZHALEH H. | AZADBAKHT M. | Issue Writer Certificate

Keywords

DEXAMETHASONEQ3

MORPHINEQ2

NEURITE ELONGATIONQ2

PC12 CELLSQ3

Abstract

BACKGROUND AND OBJECTIVE: Glucocorticoids as powerful agents that effect on cell growth, differentiation and cell death are in throughout life. MORPHINE at low concentration stimulates process elongation in neurons. In this study we examined the effects of low concentrations of MORPHINE on DEXAMETHASONE induced-cell death in differentiated PC12 CELLS.METHODS: PC12 CELLS were cultured in RPMI 1640 culture medium containing 1% FBS, 2 mm L-glutamin, 1% NEAA and 1% antibiotic penicillin/stereptomycine. Cells were treated with different concentrations of DEXAMETHASONE in the presence of 214 nM staurosporine as neuronal differentiated agent. PC12 CELLS were divided into two groups, group I, treatment with different concentrations of DEXAMETHASONE without MORPHINE and group II, treatment with different concentrations of DEXAMETHASONE together with MORPHINE (10-10 M). There were six treatments in each group: treatment 1 without (0 nM) DEXAMETHASONE and treatments 2 to 6 with adding 1 nM, 10 nM, 100 nM, 1mM and 10 mM DEXAMETHASONE in culture medium, respectively.FINDINGS: Our results showed that the cell viability was increased in group I, treatments 2, 3 and 4 and was decreased in treatments 1 and 5 and 6 (p<0.05). Cell viability was increased in group II, treatments 1, 2, 3 and 4, and was decreased in treatments 5 and 6 (p<0.05). Total neurite length in group I treatments 1-6 was decreased (148.70±2.37, 85.06±1.61, 88.38±1.58, 91.05±1.66, 72.82±1.32 and 69.18±1.18 mm) respectively (p<0.05). In group I treatments 1 and 6 had highest and lowest total neurite length, respectively (p<0.05). Total neurite length in group II treatments 1-6 were 164.22±4.45, 105.19±2.84, 108.33±3.09, 149.46±4.04, 117.60±3.08 and 115.24±2.73 mm, respectively. In group II treatment 1 had highest and treatments 2 and 3 had lowest total neurite length (p<0.05).CONCLUSION: It is concluded that low concentrations MORPHINE enhances NEURITE ELONGATION and viability in PC12 CELLS.

Cites

A STUDY OF MECHANISM AND RATE OF PC12 CANCER CELL DESTRUCTION INDUCED BY LYSINE-COATED GOLD NANOPARTICLE

References

No record.

Cite

APA: Copy

NAJMI NEJAD, S., ZHALEH, H., & AZADBAKHT, M.. (2013). PROTECTIVE EFFECTS OF MORPHINE LOW CONCENTRATION ON DEXAMETHASONE-INDUCED CELL DEATH IN DIFFERENTIATED PC12 CELLS. JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS), 15(5), 7-16. SID. https://sid.ir/paper/73839/en

Vancouver: Copy

NAJMI NEJAD S., ZHALEH H., AZADBAKHT M.. PROTECTIVE EFFECTS OF MORPHINE LOW CONCENTRATION ON DEXAMETHASONE-INDUCED CELL DEATH IN DIFFERENTIATED PC12 CELLS. JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS)[Internet]. 2013;15(5):7-16. Available from: https://sid.ir/paper/73839/en

IEEE: Copy

S. NAJMI NEJAD, H. ZHALEH, and M. AZADBAKHT, “PROTECTIVE EFFECTS OF MORPHINE LOW CONCENTRATION ON DEXAMETHASONE-INDUCED CELL DEATH IN DIFFERENTIATED PC12 CELLS,” JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS), vol. 15, no. 5, pp. 7–16, 2013, [Online]. Available: https://sid.ir/paper/73839/en

Related Journal Papers