Inhibition of herpes simplex virus type 1 replication by novel hsa-miR-7704 in vitro

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Shabani Mehdi; NASR ESFAHANI BAHRAM; Sadegh Ehdaei Bahar; MOGHIM SHARAREH; MIRZAEI AREZOO; SHARIFI MOHAMMADREZA; MOUHEBAT LEILI

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Journal: RESEARCH IN PHARMACEUTICAL SCIENCES (RPS) Year:2019 | Volume:14 | Issue:2 Page(s): 167-174

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Title

Inhibition of herpes simplex virus type 1 replication by novel hsa-miR-7704 in vitro

Author(s)

Keywords

Antiviral therapy;HSV

1;Micro RNA;miR

has

7704;miR

SX1

Abstract

Herpes simplex virus type 1 (HSV-1) infections are one of the most common diseases in human population. HSV-1 causes subclinical, mild to severe diseases, especially in immunocompromised patients. Acyclovir has been used to reduce manifestations of HSV-1 infections. The extensive use of this drug has led to the development of resistant strains. Thus, designing a novel anti-herpes drug with different mechanisms of action is urgently needed. Cellular microRNAs (miRNAs) have direct antiviral effects in addition to their regulatory functions. In this study we used a novel miRNA (hsa-miR-7704), expressed in macrophages, to inhibit HSV-1 lytic infection in HeLa cells. Synthesized hsa-miR-7704 mimics were transfected into HSV-1 infected HeLa cell. The inhibitory effects of the miRNA were evaluated by plaque assay, real time polymerase chain reaction and the viral titers were measured by the 50% tissue culture infective dose (TCID50). The viral titer and cell cytopathic effect were dramatically decreased in HeLa cells transfected with hsa-miR-7704 (50 and 100 nM), compared with HSV-1 infected cells alone or transfected with the mock miRNA control. These results suggest that hsa-miR-7704 inhibits HSV-1 replication efficiently in vitro. This may provide an alternative mechanism to prevent HSV-1 infections.

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APA: Copy

Shabani, Mehdi, NASR ESFAHANI, BAHRAM, Sadegh Ehdaei, Bahar, MOGHIM, SHARAREH, MIRZAEI, AREZOO, SHARIFI, MOHAMMADREZA, & MOUHEBAT, LEILI. (2019). Inhibition of herpes simplex virus type 1 replication by novel hsa-miR-7704 in vitro. RESEARCH IN PHARMACEUTICAL SCIENCES (RPS), 14(2), 167-174. SID. https://sid.ir/paper/738807/en

Vancouver: Copy

Shabani Mehdi, NASR ESFAHANI BAHRAM, Sadegh Ehdaei Bahar, MOGHIM SHARAREH, MIRZAEI AREZOO, SHARIFI MOHAMMADREZA, MOUHEBAT LEILI. Inhibition of herpes simplex virus type 1 replication by novel hsa-miR-7704 in vitro. RESEARCH IN PHARMACEUTICAL SCIENCES (RPS)[Internet]. 2019;14(2):167-174. Available from: https://sid.ir/paper/738807/en

IEEE: Copy

Mehdi Shabani, BAHRAM NASR ESFAHANI, Bahar Sadegh Ehdaei, SHARAREH MOGHIM, AREZOO MIRZAEI, MOHAMMADREZA SHARIFI, and LEILI MOUHEBAT, “Inhibition of herpes simplex virus type 1 replication by novel hsa-miR-7704 in vitro,” RESEARCH IN PHARMACEUTICAL SCIENCES (RPS), vol. 14, no. 2, pp. 167–174, 2019, [Online]. Available: https://sid.ir/paper/738807/en

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