Apoptosis induction and proliferation inhibition by silibinin encapsulated in nanoparticles in MIA PaCa-2 cancer cells and deregulation of some miRNAs

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Khakinezhad Tehrani Fatemeh; RANJI NAJMEH; KOUHKAN FATEMEH; HOSSEINZADEH SIMZAR

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Journal: Iranian Journal of Basic Medical Sciences Year:2020 | Volume:23 | Issue:4 Page(s): 469-482

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Title

Apoptosis induction and proliferation inhibition by silibinin encapsulated in nanoparticles in MIA PaCa-2 cancer cells and deregulation of some miRNAs

Author(s)

Khakinezhad Tehrani Fatemeh | RANJI NAJMEH | KOUHKAN FATEMEH | HOSSEINZADEH SIMZAR | Issue Writer Certificate

Keywords

Cancer stem cell

miRNA

Nanoparticles

Pancreatic cancer

Silibinin

Abstract

Objective(s): Silibinin, as an herbal compound, has anti-cancer activity. Because of low solubility of Silibinin in water and body fluids, it was encapsulated in polymersome Nanoparticles and its effects were evaluated on Pancreatic cancer cells and Cancer stem cells. Materials and Methods: MIA PaCa-2 Pancreatic cancer cells were treated with different doses of Silibinin encapsulated in polymersome Nanoparticles (SPNs). Stemness of MIA PaCa-2 cells was evaluated by hanging drop technique and CD133, CD24, and CD44 staining. The effects of SPNs on cell cycle, apoptosis and the expression of several genes and miRNAs were investigated. Results: IC50 of SPNs was determined to be 40 μ g/ml after 24 hr. Our analysis showed that >98% of MIA PaCa-2 cells expressed three stem cell markers. FACS analysis showed a decrease in these markers in SPNs-treated cells. PI/AnnexinV staining revealed that 40 μ g/ml and 50 μ g/ml of SPNs increased apoptosis up to ~40% and >80% of treated cells, respectively. Upregulation of miR-34a, miR-126, and miR-let7b and downregulation of miR-155, miR-222 and miR-21 was observed in SPNs-treated cells. In addition, downregulation of some genes involved in proliferation or migration such as AKT3, MASPINE, and SERPINEA12, and upregulation of apoptotic genes were observed in treated cells. Conclusion: Our results suggested that SPNs induced apoptosis and inhibited migration and proliferation in pancreatic cells and Cancer stem cells through suppression of some onco-miRs and induction of some tumor suppressive miRs, as well as their targets.

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APA: Copy

Khakinezhad Tehrani, Fatemeh, RANJI, NAJMEH, KOUHKAN, FATEMEH, & HOSSEINZADEH, SIMZAR. (2020). Apoptosis induction and proliferation inhibition by silibinin encapsulated in nanoparticles in MIA PaCa-2 cancer cells and deregulation of some miRNAs. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 23(4), 469-482. SID. https://sid.ir/paper/738825/en

Vancouver: Copy

Khakinezhad Tehrani Fatemeh, RANJI NAJMEH, KOUHKAN FATEMEH, HOSSEINZADEH SIMZAR. Apoptosis induction and proliferation inhibition by silibinin encapsulated in nanoparticles in MIA PaCa-2 cancer cells and deregulation of some miRNAs. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2020;23(4):469-482. Available from: https://sid.ir/paper/738825/en

IEEE: Copy

Fatemeh Khakinezhad Tehrani, NAJMEH RANJI, FATEMEH KOUHKAN, and SIMZAR HOSSEINZADEH, “Apoptosis induction and proliferation inhibition by silibinin encapsulated in nanoparticles in MIA PaCa-2 cancer cells and deregulation of some miRNAs,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 23, no. 4, pp. 469–482, 2020, [Online]. Available: https://sid.ir/paper/738825/en

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