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Information Journal Paper

Title

Bone marrow dendritic cells deficient for CD40 and IL-23p19 are tolerogenic in vitro

Pages

  287-292

Abstract

 Objective(s): In addition to pro-inflammatory role, dendritic cells (DCs) can also be anti-inflammatory when they acquire tolerogenic phenotype. In this study we tested the role of CD40 and IL-23p19 in antigen presenting function of bone marrow-derived DCs (BMDCs) by comparing BMDCs derived from CD40 knockout (CD40KO-DCs) and IL-23p19 (IL-23p19KO-DCs) knockout mice with those from C57BL/6 mice (Cont-DCs). We have focused on CD40 and IL-23, as these molecules have well established pro-inflammatory roles in a number of autoimmune and inflammatory diseases. Materials and Methods: The expression of maturation markers MHC II and co-stimulatory molecules CD40, CD80, and CD86 was analyzed by flow cytometry, while the expression of CD40 and IL-23p19 was measured by RT-PCR. The capacity of BMDCs to activate CD4+ T cells was evaluated by 3H-thymidine incorporation, and the capacity of BMDCs to uptake antigen was evaluated using fluorescent OVA and flow cytometry. Results: The lack of CD40 or IL-23p19 had no effect on uptake of FITC-OVA by the DCs, confirming their immature phenotype. Moreover, CD40KO-DCs had significantly reduced capacity to stimulate proliferation of CD4+ T cells. CD4+ T cells activated by CD40KO-DCs and IL-23p19KO-DCs produced significantly less IFN-γ (P-value ≤ 0. 05), while CD4+ T cells stimulated by IL-23p19KO-DCs produced less GM-CSF and more IL-10 than Cont-DCs. Conclusion: This study shows that CD40KO-DCs and IL-23p19KO-DCs have a marked tolerogenic potency in vitro. Future in vivo studies should determine if and to what extent DCs lacking CD40 or IL-23 have a potential to be useful in therapy of autoimmune inflammation.

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    APA: Copy

    Ciric, Bogoljub, KAMALI SARVESTANI, ESKANDAR, & Rostami, Abdolmohamad. (2020). Bone marrow dendritic cells deficient for CD40 and IL-23p19 are tolerogenic in vitro. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 23(3), 287-292. SID. https://sid.ir/paper/739049/en

    Vancouver: Copy

    Ciric Bogoljub, KAMALI SARVESTANI ESKANDAR, Rostami Abdolmohamad. Bone marrow dendritic cells deficient for CD40 and IL-23p19 are tolerogenic in vitro. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2020;23(3):287-292. Available from: https://sid.ir/paper/739049/en

    IEEE: Copy

    Bogoljub Ciric, ESKANDAR KAMALI SARVESTANI, and Abdolmohamad Rostami, “Bone marrow dendritic cells deficient for CD40 and IL-23p19 are tolerogenic in vitro,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 23, no. 3, pp. 287–292, 2020, [Online]. Available: https://sid.ir/paper/739049/en

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