Evaluation of apamin effects on myelination process in C57BL/6 mice model of multiple sclerosis

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Mohammadi Rad Maedeh; GHASEMI NAZEM; Aliomrani Mehdi

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: RESEARCH IN PHARMACEUTICAL SCIENCES (RPS) Year:2019 | Volume:14 | Issue:5 Page(s): 424-431

Download Full-Text

View:

108

Download:

Cites:

Information Journal Paper

Title

Evaluation of apamin effects on myelination process in C57BL/6 mice model of multiple sclerosis

Author(s)

Mohammadi Rad Maedeh | GHASEMI NAZEM | Aliomrani Mehdi | Issue Writer Certificate

Keywords

Apamin

C57BL/6

Cuprizone

Multiple sclerosis

Myelin

Abstract

Multiple sclerosis (MS) is a deMyelinating disease that causes chronic inflammation in the central nervous system. The aim of this study was to investigate the effects of Apamin administration on Myelination process. MS was induced by feeding Cuprizone pellets (0. 2%) for 6 weeks (deMyelination phase) followed by normal feeding for additional 2 weeks (reMyelination phase). Briefly, C57BL/6 male mice were randomly divided into six groups. Group 1, received the regular food pellets. Group 2 contained two subgroups of 6 animals each (n = 2 × 6). First group received Cuprizone for 6 weeks and the sacrificed while the second group after 6 weeks of Cuprizone, received no treatment for additional 2 weeks. Group 3 (co-treatment group) was composed of two subgroups of 6 animals each (n = 2 × 6). Both subgroups received Apamin (100 μ g/kg) intraperitoneally twice a week for 6 weeks. First subgroup terminated at this time and the second subgroup was fed normal diet for two additional weeks. Group 4 (post-treatment, n = 6) received Apamin (100 μ g/kg) intraperitoneally twice a week for 2 weeks after Cuprizone secession. Groups 5 and 6 (vehicle, n = 6 in each group) received phosphate buffered saline as the vehicle of Apamin during deMyelination and reMyelination phase. At the end of each phase, mice were deeply anesthetized and perfused. Groups 5 and 6 (vehicle) received PBS as the vehicle during both phases. Mice were anesthetized, perfused with PBS through their heart, and their brains were removed. Brain sections stained with luxol fast blue and the images were analyzed. Apamin co-treatment significantly increased the Myelin content as compared to the Cuprizone group. Also, mild elevation in the Myelinated areas was observed with Apamin post-treatment in comparison with reMyelination phase. Our results revealed that Apamin prevents Myelin destruction more significantly as compared to reMyelination process. This observation explains the possible role of Apamin in inhibiting the activation of the microglia cells than stimulation of the oligodendrocytic precursor cells.

Cites

No record.

References

No record.

Cite

APA: Copy

Mohammadi Rad, Maedeh, GHASEMI, NAZEM, & Aliomrani, Mehdi. (2019). Evaluation of apamin effects on myelination process in C57BL/6 mice model of multiple sclerosis. RESEARCH IN PHARMACEUTICAL SCIENCES (RPS), 14(5), 424-431. SID. https://sid.ir/paper/739063/en

Vancouver: Copy

Mohammadi Rad Maedeh, GHASEMI NAZEM, Aliomrani Mehdi. Evaluation of apamin effects on myelination process in C57BL/6 mice model of multiple sclerosis. RESEARCH IN PHARMACEUTICAL SCIENCES (RPS)[Internet]. 2019;14(5):424-431. Available from: https://sid.ir/paper/739063/en

IEEE: Copy

Maedeh Mohammadi Rad, NAZEM GHASEMI, and Mehdi Aliomrani, “Evaluation of apamin effects on myelination process in C57BL/6 mice model of multiple sclerosis,” RESEARCH IN PHARMACEUTICAL SCIENCES (RPS), vol. 14, no. 5, pp. 424–431, 2019, [Online]. Available: https://sid.ir/paper/739063/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops