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Information Journal Paper

Title

Specific Detection of Fasciola hepatica and F. gigantica in Infected Domesticated Animals Using High-Resolution Melting Analysis (HRM)

Pages

  521-529

Keywords

Polymerase chain reaction (PCR) 

Abstract

 Background: It is difficult to make an exact morphological distinction between Fasciola hepatica and Fasciola gigantica. We used High Resolution Melting analysis (HRM) method to differentiate the F. hepatica species from F. gigantica in order to differentiate them. Methods: Overall, 80 adult liver flukes were collected from infected slaughtered animals including cattle, sheep and goats from Lorestan Province, western Iran from Sep 2015 to Aug 2017. Genomic DNA was extracted using commercial DNA extraction kit. The multilocus sequences of mDNA including COX1, COX3 and ND6 were amplified employing real-time PCR & HRM analysis. Specific and universal primer pairs were designed for differentiation Fasciola spp. Results: Universal primers cannot be used to distinguish between these two species, but in the contrary, specific primer pairs of each species could differentiate them properly. Molecular identification using specific primer pairs were consistent. Conclusion: HRM is a simple, fast and reliable method for detecting and differentiating F. hepatica from F. gigantica and can be used for diagnostic and epidemiological purposes.

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    • Cite

    APA: Copy

    MOGHADAMIZAD, Zeinab, HOSSEINI SAFA, AHMAD, MOHEBALI, MEHDI, Heydarian, Peyman, ARYAEIPOUR, MOJGAN, & ROKNI, MOHAMMAD BAGHER. (2020). Specific Detection of Fasciola hepatica and F. gigantica in Infected Domesticated Animals Using High-Resolution Melting Analysis (HRM). IRANIAN JOURNAL OF PUBLIC HEALTH, 49(3), 521-529. SID. https://sid.ir/paper/740177/en

    Vancouver: Copy

    MOGHADAMIZAD Zeinab, HOSSEINI SAFA AHMAD, MOHEBALI MEHDI, Heydarian Peyman, ARYAEIPOUR MOJGAN, ROKNI MOHAMMAD BAGHER. Specific Detection of Fasciola hepatica and F. gigantica in Infected Domesticated Animals Using High-Resolution Melting Analysis (HRM). IRANIAN JOURNAL OF PUBLIC HEALTH[Internet]. 2020;49(3):521-529. Available from: https://sid.ir/paper/740177/en

    IEEE: Copy

    Zeinab MOGHADAMIZAD, AHMAD HOSSEINI SAFA, MEHDI MOHEBALI, Peyman Heydarian, MOJGAN ARYAEIPOUR, and MOHAMMAD BAGHER ROKNI, “Specific Detection of Fasciola hepatica and F. gigantica in Infected Domesticated Animals Using High-Resolution Melting Analysis (HRM),” IRANIAN JOURNAL OF PUBLIC HEALTH, vol. 49, no. 3, pp. 521–529, 2020, [Online]. Available: https://sid.ir/paper/740177/en

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