PTPN22 Silencing in Human Acute T-Cell Leukemia Cell Line (Jurkat Cell) and its Effect on the Expression of miR-181a and miR-181b

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Baghbani Elham; KHAZE VAHID; SADREDDINI SANAM; MOKHTARZADEH AHAD; MANSOORI BEHZAD; MOHAMMADI ALI; Vatankhahan Vida; Toosi Parisa; BARADARAN BEHZAD

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Advanced Pharmaceutical Bulletin Year:2018 | Volume:8 | Issue:2 Page(s): 277-282

Download Full-Text

View:

115

Download:

113

Cites:

Information Journal Paper

Title

PTPN22 Silencing in Human Acute T-Cell Leukemia Cell Line (Jurkat Cell) and its Effect on the Expression of miR-181a and miR-181b

Author(s)

Keywords

T-ALL

PTPN22

miR-181a

miR-181b

Abstract

Purpose: T-cell acute lymphoblastic leukemia (T-ALL) is one of the most common malignancies associated with T-lymphocytes, accounting for 10 to 15 percent of ALL cases in children and 25 percent in adults. Innovative therapeutic approaches that overcome ineffective treatments on tumor cells may be a potential source of improvement in therapeutic approaches. Suppression of gene expression at transfusion level is one of the important strategies in gene therapy. The expression of PTPN22 and miR-181 genes in all types of hematologic malignancies increases and is likely to contribute to the survival and death of cells by affecting a variety of signaling pathways. The purpose of this study was to determine the role of PTPN22 inhibition by siRNA, and alteration in miR-181a and miR-181b in Jurkat cell line. Methods: Jurkat cells were transfected with 80 pmol of siRNA to inhibit PTPN22. After that, expression of PTPN22 mRNA and transcript levels of miR-181a and miR-181b were measured with Real-time PCR after 48hrs. Results: Experiments demonstrated that siRNA transfection resulted in significant downregulation of PTPN22 mRNA after 48 hrs in 80 pmol dose of siRNA. Moreover, transcript levels of both miR-181a and miR-181b was decreased after transfection. Conclusion: PTPN22, miR-181a and miR-181b might be involved in progression of Jurkat cells and targeting these molecules by RNAi might confer promising tool in treatment of T-ALL.

Cites

No record.

References

No record.

Cite

APA: Copy

Baghbani, Elham, KHAZE, VAHID, SADREDDINI, SANAM, MOKHTARZADEH, AHAD, MANSOORI, BEHZAD, MOHAMMADI, ALI, Vatankhahan, Vida, Toosi, Parisa, & BARADARAN, BEHZAD. (2018). PTPN22 Silencing in Human Acute T-Cell Leukemia Cell Line (Jurkat Cell) and its Effect on the Expression of miR-181a and miR-181b. ADVANCED PHARMACEUTICAL BULLETIN, 8(2), 277-282. SID. https://sid.ir/paper/745768/en

Vancouver: Copy

Baghbani Elham, KHAZE VAHID, SADREDDINI SANAM, MOKHTARZADEH AHAD, MANSOORI BEHZAD, MOHAMMADI ALI, Vatankhahan Vida, Toosi Parisa, BARADARAN BEHZAD. PTPN22 Silencing in Human Acute T-Cell Leukemia Cell Line (Jurkat Cell) and its Effect on the Expression of miR-181a and miR-181b. ADVANCED PHARMACEUTICAL BULLETIN[Internet]. 2018;8(2):277-282. Available from: https://sid.ir/paper/745768/en

IEEE: Copy

Elham Baghbani, VAHID KHAZE, SANAM SADREDDINI, AHAD MOKHTARZADEH, BEHZAD MANSOORI, ALI MOHAMMADI, Vida Vatankhahan, Parisa Toosi, and BEHZAD BARADARAN, “PTPN22 Silencing in Human Acute T-Cell Leukemia Cell Line (Jurkat Cell) and its Effect on the Expression of miR-181a and miR-181b,” ADVANCED PHARMACEUTICAL BULLETIN, vol. 8, no. 2, pp. 277–282, 2018, [Online]. Available: https://sid.ir/paper/745768/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops