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Information Journal Paper

Title

Effect of Human Testicular Cells Conditioned Medium on In Vitro Maturation and Morphology of Mouse Oocytes

Pages

  176-184

Abstract

 Background: Testicular cell conditioned medium (TCCM) has been shown to induce female germ cell development in vitro from embryonic stem cells (ESCs). Testicular cells (TCs) secrete a variety of growth factors such as growth differentiation factor-9 (GDF-9), bone morphogenetic protein 4 (BMP-4), stem cell factor (SCF), leukemia inhibitory factor (LIF), and other, that could improve oocyte maturation. Here we have investigated the effect of human TCCM (hTCCM) on in vitro maturation (IVM) and morphology of mouse oocytes. Materials and Methods: In this experimental study, 360 germinal vesicle (GV) oocytes were obtained from NMRI mice, aged 4-6 weeks that had received 5 IU pregnant mare's serum gonadotropin (PMSG) 48 hours before. GV oocytes were subjected to IVM. 120 GV oocytes were cultured in each medium; hTCCM as the test group, DMEM + 20%FBS as the control group and Ham’ s F10 + HFF medium as the sham group. The rates of the IVM and perivitelline space (PVS) changes were recorded at 8, 16 and 24 hours after culture. The metaphase II (MII) oocytes were subjected for in vitro fertilization (IVF) and the fertilization rate was evaluated after 1, 2, and 3 days. Results: There was a significant difference between the maturation rates in hTCCM (31. 67% MII) and the control [0% MII, P<0. 05, (7. 5% MI, 52. 5% deg. and 40%GV)] groups but there was not a significant difference between the maturation rates in hTCCM and the sham group (53. 33% MII, P>0. 05). IVF success rate for MII oocytes obtained from IVM in the hTCCM group was 28. 94% (n=11). Our data showed that hTCCM is an effective medium for GV oocyte growth and maturation compared to the control medium.

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    APA: Copy

    ADIB, MARYAM, SEIFATI, SEYED MORTEZA, & DEHGHANI ASHKEZARI, MAHMOOD. (2020). Effect of Human Testicular Cells Conditioned Medium on In Vitro Maturation and Morphology of Mouse Oocytes. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, 14(3), 176-184. SID. https://sid.ir/paper/746683/en

    Vancouver: Copy

    ADIB MARYAM, SEIFATI SEYED MORTEZA, DEHGHANI ASHKEZARI MAHMOOD. Effect of Human Testicular Cells Conditioned Medium on In Vitro Maturation and Morphology of Mouse Oocytes. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY[Internet]. 2020;14(3):176-184. Available from: https://sid.ir/paper/746683/en

    IEEE: Copy

    MARYAM ADIB, SEYED MORTEZA SEIFATI, and MAHMOOD DEHGHANI ASHKEZARI, “Effect of Human Testicular Cells Conditioned Medium on In Vitro Maturation and Morphology of Mouse Oocytes,” INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, vol. 14, no. 3, pp. 176–184, 2020, [Online]. Available: https://sid.ir/paper/746683/en

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