Anti-inflammatory activity of Echium amoenum extract on macrophages mediated by inhibition of inflammatory mediators and cytokines expression

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Naseri najme; KALANTAR Kurosh; AMIRGHOFRAN ZAHRA

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: RESEARCH IN PHARMACEUTICAL SCIENCES (RPS) Year:2018 | Volume:13 | Issue:1 Page(s): 73-81

Download Full-Text

View:

130

Download:

Cites:

Information Journal Paper

Title

Anti-inflammatory activity of Echium amoenum extract on macrophages mediated by inhibition of inflammatory mediators and cytokines expression

Author(s)

Naseri najme | KALANTAR Kurosh | AMIRGHOFRAN ZAHRA | Issue Writer Certificate

Keywords

Echium amoenum

Macrophages

Anti-inflammatory

Abstract

Echium amoenum (Boraginaceae) is an important remedy used for various illnesses. In this study, we investigated the Anti-inflammatory effects of E. amoenum in the J774. 1A macrophage cell line. We prepared ethyl acetate, dichloromethane and hexane extracts from E. amoenum flowers and examined their possible cytotoxic effects using MTT assay. Lipopolysaccharide (LPS)-stimulated Macrophages were treated with the extracts after which we measured nitric oxide (NO) production by Griess method. Inducible NO synthase (iNOS), cyclooxygenase-2 (COX2), tumor necrosis factor (TNF)-α , interleukin (IL)-1β , and IL-6 gene expressions were examined by real time-PCR. IL-1β and IL-6 cytokine levels were measured by enzymelinked immunosorbent assay (ELISA). The hexane extract with a half maximal inhibitory concentration (IC50) of 39. 8 μ g/mL most effectively reduced NO production. Real time-PCR analysis indicated reduced levels of iNOS ((0. 05 ± 0. 006 relative fold change (RFC)) and COX2 (0. 06 ± 0. 002 RFC) gene expressions with the 100 μ g/mL hexane extract (P < 0. 001). IL1-β , TNF-α , and IL-6 gene expression levels decreased at all concentrations of the extract (less than ≈ 0. 28 RFC). Treatment of LPS-stimulated cells with 100 μ g/mL of the extract reduced IL-1β secretion to 27. 9 ± 0. 21 pg/mL and IL-6 to 555 ± 166 pg/mL. In conclusion, E. amoenum hexane extract showed the greatest reduction in macrophage NO secretion compared to other extracts. This extract could modulate the inflammatory mode of the Macrophages by causing reductions in iNOS and COX2 enzymes as well as IL-1β , IL-6, and TNF-α cytokine levels. The results of this study have shown the Anti-inflammatory effects of this plant. Further studies regarding its therapeutic potential in inflammatory disorders are recommended.

Cites

References

No record.

Cite

APA: Copy

Naseri, najme, KALANTAR, Kurosh, & AMIRGHOFRAN, ZAHRA. (2018). Anti-inflammatory activity of Echium amoenum extract on macrophages mediated by inhibition of inflammatory mediators and cytokines expression. RESEARCH IN PHARMACEUTICAL SCIENCES (RPS), 13(1), 73-81. SID. https://sid.ir/paper/747903/en

Vancouver: Copy

Naseri najme, KALANTAR Kurosh, AMIRGHOFRAN ZAHRA. Anti-inflammatory activity of Echium amoenum extract on macrophages mediated by inhibition of inflammatory mediators and cytokines expression. RESEARCH IN PHARMACEUTICAL SCIENCES (RPS)[Internet]. 2018;13(1):73-81. Available from: https://sid.ir/paper/747903/en

IEEE: Copy

najme Naseri, Kurosh KALANTAR, and ZAHRA AMIRGHOFRAN, “Anti-inflammatory activity of Echium amoenum extract on macrophages mediated by inhibition of inflammatory mediators and cytokines expression,” RESEARCH IN PHARMACEUTICAL SCIENCES (RPS), vol. 13, no. 1, pp. 73–81, 2018, [Online]. Available: https://sid.ir/paper/747903/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops