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Title

Conditioned Medium from Cultured Colorectal Cancer Cells Affects Peripheral Blood Mononuclear Cells Inflammatory Phenotype in Vitro

Pages

  334-341

Abstract

 Background: Colorectal cancer (CRC) is the third most common cancer worldwide. Studies have indicated that immune cells and soluble factors play a key role in maintaining the balance between tumor-promoting inflammation and anti-tumor immunity. It has been shown that secreted Cytokines from CRC cell lines could affect peripheral blood mononuclear cells (PBMCs), Monocytes, and Macrophages phenotypes. Macrophage infiltration has been associated with good prognosis in some cancers, but with poor prognosis in others. The present study aimed to evaluate the effect of conditioned media from CRC cells (Caco-2) on immune responses produced by PBMCs. Methods: The present study was performed at the Gastroenterology and Liver Diseases Research Institute, Shahid Beheshti University of Medical Sciences (Tehran, Iran) in 2017. Human Monocytes were isolated from PBMCs by Ficoll gradient media. The co-culture of Monocytes and Caco-2 conditioned media was carried out. RNA extraction and cDNA synthesis of Monocytes were performed after 96 hours. Gene expression of pro-and anti-inflammatory Cytokines was evaluated by realtime PCR. Statistical analysis was performed using the SPSS software (version 21. 0) with the independent sample t test. P<0. 05 was considered statistically significant. Results: Compared to the control group, the treated Monocytes showed increased levels of interleukin-6 (P=0. 001), interleukin-12b (P=0. 001), and interferon-gamma (P=0. 02), as well as decreased amounts of interleukin-4 (P=0. 01), interleukin-10 (P=0. 01), and tumor necrosis factor-alpha (P=0. 01). Conclusion: Secreted Cytokines and soluble factors from Caco-2 induced the differentiation of PBMCs, particularly the Monocytes, toward inflammatory phenotype according to the altered gene expression of inflammatory and anti-inflammatory Cytokines.

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    APA: Copy

    Ashtibaghaei, Kaveh, HASHEMI, MEHRDAD, HASHEMI, MAHMOUD, ASADZADEH AGHDAEI, HAMID, & ZALI, MOHAMMAD REZA. (2019). Conditioned Medium from Cultured Colorectal Cancer Cells Affects Peripheral Blood Mononuclear Cells Inflammatory Phenotype in Vitro. IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS), 44(4), 334-341. SID. https://sid.ir/paper/748704/en

    Vancouver: Copy

    Ashtibaghaei Kaveh, HASHEMI MEHRDAD, HASHEMI MAHMOUD, ASADZADEH AGHDAEI HAMID, ZALI MOHAMMAD REZA. Conditioned Medium from Cultured Colorectal Cancer Cells Affects Peripheral Blood Mononuclear Cells Inflammatory Phenotype in Vitro. IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS)[Internet]. 2019;44(4):334-341. Available from: https://sid.ir/paper/748704/en

    IEEE: Copy

    Kaveh Ashtibaghaei, MEHRDAD HASHEMI, MAHMOUD HASHEMI, HAMID ASADZADEH AGHDAEI, and MOHAMMAD REZA ZALI, “Conditioned Medium from Cultured Colorectal Cancer Cells Affects Peripheral Blood Mononuclear Cells Inflammatory Phenotype in Vitro,” IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS), vol. 44, no. 4, pp. 334–341, 2019, [Online]. Available: https://sid.ir/paper/748704/en

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