مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

SDF-1α Reduces Human Natural Killer Cell Cytotoxicity against Chronic Myelogenous Leukemia K562 Cells

Pages

  493-500

Abstract

 Stromal cell-derived factor-1 alpha (SDF-1α; ) has been shown to be up-regulated in a variety of malignancies. So that, its expression is associated with poor prognosis and invasiveness. Natural killer (NK) cells are important effector cells against virus-infected and transformed cells. Especially they play a key role in tumor immune surveillance. Whereas it was not well understood whether SDF-1α; modulates anti-tumor immune response or not, the purpose of the present study was to investigate the effect of SDF-1α; on the cytotoxic properties of peripheral blood NK cells. Human peripheral blood NK cells were freshly isolated using MACSxpess system and cultured in the presence or absence of recombinant human SDF-1α; or SDF-1α; plus CXCR4 antagonist, AMD3100. CD107a degranulation assay was conducted through the co-culture of NK cells with K562 cells. The percentage of CD107a positive cells was assessed by flowcytometry. Effect of SDF-1α; was also examined on the mRNA levels of NKG2A and NKG2D as indicator examples of NK cell inhibitory and activating receptors, respectively. SDF-1α; significantly decreased the degranulation activity of NK cells (p=0. 04). The mRNA content of NKG2D was down-regulated under the influence of SDF-1α; (p=0. 03). Moreover, AMD3100 exhibited a trend in recovering the NKG2D mRNA level to its un-treated state (p=0. 05). The present study reveals that SDF-1α; has a negative impact on NK cell activity and might is involved in tumor immune-suppression. Thus, it can be concluded that microenvironment manipulations targeting SDF-1α; may reinforce current cancer therapies by disturbing one of the immune-suppressive axes in the cancerous milieu.

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    APA: Copy

    Mardomi, Alireza, HOSSEIN NATAJ, HADI, JAFARI, NARJES, & ABEDIANKENARI, SAEID. (2019). SDF-1α Reduces Human Natural Killer Cell Cytotoxicity against Chronic Myelogenous Leukemia K562 Cells. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), 18(5), 493-500. SID. https://sid.ir/paper/750602/en

    Vancouver: Copy

    Mardomi Alireza, HOSSEIN NATAJ HADI, JAFARI NARJES, ABEDIANKENARI SAEID. SDF-1α Reduces Human Natural Killer Cell Cytotoxicity against Chronic Myelogenous Leukemia K562 Cells. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI)[Internet]. 2019;18(5):493-500. Available from: https://sid.ir/paper/750602/en

    IEEE: Copy

    Alireza Mardomi, HADI HOSSEIN NATAJ, NARJES JAFARI, and SAEID ABEDIANKENARI, “SDF-1α Reduces Human Natural Killer Cell Cytotoxicity against Chronic Myelogenous Leukemia K562 Cells,” IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), vol. 18, no. 5, pp. 493–500, 2019, [Online]. Available: https://sid.ir/paper/750602/en

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