Comparison of E Test and Disc Diffusion Methods for Susceptibility Testing of Filamentous Fungi; Experience of a Routine Lab

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Hassan Reem Mostafa; Ismail Dalia Kadry; Samy Elkholy Yasmine

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Journal Paper

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Journal: Archives of Clinical Infectious Diseases Year:2018 | Volume:13 | Issue:3 Page(s): 0-0

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Information Journal Paper

Title

Comparison of E Test and Disc Diffusion Methods for Susceptibility Testing of Filamentous Fungi; Experience of a Routine Lab

Author(s)

Hassan Reem Mostafa | Ismail Dalia Kadry | Samy Elkholy Yasmine | Issue Writer Certificate

Keywords

Aspergillus

Fungal Drug Sensitivity Tests

Diffusion

Fungi

Abstract

Background: Testing for antifungal susceptibility to newly introduced drugs is standardized through the clinical laboratory standard institute (CLSI) broth micro-dilution method for testing of molds (M38-A2), yet is difficult to use routinely. Objectives: To compare two agar-based Diffusion methods on two types of media for fungal drug sensitivity testing. Materials and Methods: The E-test method and the disc Diffusion method were used on non-supplemented Muller Hinton agar (MHA) and RPMI for evaluating the in-vitro susceptibility of 48 clinical isolates of filamentous Fungi to amphotericin B, itraconazole, voriconazole, and caspoFungin. Results: Categorical agreement between E-test and disc Diffusion method for itraconazole and voriconazole was 100%, yet for amphotericin B on MHA agar was 66. 67%, and on RPMI, it was 47. 92%. The correlation coefficient (R) between the inhibition zone diameters when using MHA and RPMI for itraconazole, voriconazole, amphotericin B and caspoFungin was +0. 745, +0. 901 +0. 649, and +0. 409, respectively. Conclusions: Routine antifungal susceptibility testing using disk Diffusion can be implemented in routine microbiology work up in limited resources.

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APA: Copy

Hassan, Reem Mostafa, Ismail, Dalia Kadry, & Samy Elkholy, Yasmine. (2018). Comparison of E Test and Disc Diffusion Methods for Susceptibility Testing of Filamentous Fungi; Experience of a Routine Lab. ARCHIVES OF CLINICAL INFECTIOUS DISEASES, 13(3), 0-0. SID. https://sid.ir/paper/753304/en

Vancouver: Copy

Hassan Reem Mostafa, Ismail Dalia Kadry, Samy Elkholy Yasmine. Comparison of E Test and Disc Diffusion Methods for Susceptibility Testing of Filamentous Fungi; Experience of a Routine Lab. ARCHIVES OF CLINICAL INFECTIOUS DISEASES[Internet]. 2018;13(3):0-0. Available from: https://sid.ir/paper/753304/en

IEEE: Copy

Reem Mostafa Hassan, Dalia Kadry Ismail, and Yasmine Samy Elkholy, “Comparison of E Test and Disc Diffusion Methods for Susceptibility Testing of Filamentous Fungi; Experience of a Routine Lab,” ARCHIVES OF CLINICAL INFECTIOUS DISEASES, vol. 13, no. 3, pp. 0–0, 2018, [Online]. Available: https://sid.ir/paper/753304/en

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