Cloning and Expression of HP0242 Hypothetical Gene as a DNA Vaccine Candidate for Helicobacter pylori and Study of Its Immunoreactivity

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

BAGHERI ZAHRA; GHASEMIAN ABDOLMAJID; SHOKOUHI MOSTAFAVI SEYYED KHALIL; DOOSTI ABBAS

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Archives of Clinical Infectious Diseases Year:2018 | Volume:13 | Issue:1 Page(s): 0-0

Download Full-Text

View:

145

Download:

Cites:

Information Journal Paper

Title

Cloning and Expression of HP0242 Hypothetical Gene as a DNA Vaccine Candidate for Helicobacter pylori and Study of Its Immunoreactivity

Author(s)

BAGHERI ZAHRA | GHASEMIAN ABDOLMAJID | SHOKOUHI MOSTAFAVI SEYYED KHALIL | DOOSTI ABBAS | Issue Writer Certificate

Keywords

DNA Vaccine

HP0242 hypothetical Gene

immunogenicity

Helicobacter pylori

Abstract

Background: The HP0242 plays an important role in physiology of H. pylori in acidic condition. Objectives: The aim of this study was cloning and expression of immunogenicity of HP0242 gene of H. pylori. Methods: The whole sequence of the hypothetical gene was amplified with specific primers. The DNA sequence (716 bp) was cloned into E. coli TOP10F by utilizing PEGFP-C1 and PTZ vectors. The protein product in CHO cells was elucidated with the SDS-PAGE technique. Western blot analysis showed that HP0242 expressed by recombinant E. coli had favorable immune-reactivity. Western blot was conducted to assess the immune-reactivity. Results: Presence of hypothetical gene in the expression PEGFP-C1 vector was confirmed with digestion by sacII and smaI. Finally, the expression of the hypothetical protein in CHO cells was confirmed by SDS-PAGE and thus, can be considered as a DNA Vaccine candidate for H. pylori. Western blot analysis showed that HP0242 expressed by recombinant E. coli had a favorable immune-reactivity. Conclusion: The expressed HP0242 gene showed the possibility that it can be used as a candidate DNA Vaccine for H. pylori in the future research.

Cites

No record.

References

No record.

Cite

APA: Copy

BAGHERI, ZAHRA, GHASEMIAN, ABDOLMAJID, SHOKOUHI MOSTAFAVI, SEYYED KHALIL, & DOOSTI, ABBAS. (2018). Cloning and Expression of HP0242 Hypothetical Gene as a DNA Vaccine Candidate for Helicobacter pylori and Study of Its Immunoreactivity. ARCHIVES OF CLINICAL INFECTIOUS DISEASES, 13(1), 0-0. SID. https://sid.ir/paper/753796/en

Vancouver: Copy

BAGHERI ZAHRA, GHASEMIAN ABDOLMAJID, SHOKOUHI MOSTAFAVI SEYYED KHALIL, DOOSTI ABBAS. Cloning and Expression of HP0242 Hypothetical Gene as a DNA Vaccine Candidate for Helicobacter pylori and Study of Its Immunoreactivity. ARCHIVES OF CLINICAL INFECTIOUS DISEASES[Internet]. 2018;13(1):0-0. Available from: https://sid.ir/paper/753796/en

IEEE: Copy

ZAHRA BAGHERI, ABDOLMAJID GHASEMIAN, SEYYED KHALIL SHOKOUHI MOSTAFAVI, and ABBAS DOOSTI, “Cloning and Expression of HP0242 Hypothetical Gene as a DNA Vaccine Candidate for Helicobacter pylori and Study of Its Immunoreactivity,” ARCHIVES OF CLINICAL INFECTIOUS DISEASES, vol. 13, no. 1, pp. 0–0, 2018, [Online]. Available: https://sid.ir/paper/753796/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops