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Information Journal Paper

Title

A rapid method for separating and concentration of food-borne pathogens using elution from ready-to-eat vegetables

Pages

  385-393

Abstract

 Background and Objectives: Traditional culture methods for detection of Food-borne pathogens, a major public health problem, are simple, easily adaptable and very practical, but they can be laborious and time consuming. In this study, we eliminated culturing steps by developing a new separation method and therefore, decreased the detection time of Food-borne pathogens (Salmonella enterica serovar Typhimurium, Escherichia coli O157: H7 and Listeria monocytogenes) to a few hours. Materials and Methods: We used alkaline water and different alkaline buffers to elute bacteria from the lettuce surface as a model for Ready-to-eat vegetables. Buffers used were as follows: 1) 0. 05 M glycine; 2) 0. 05 M glycine-100 mM Tris base-1% (w/v) beef extract; 3) buffer peptone water; 4) buffer phosphate saline. Buffers were adjusted to pH of 9, 9. 5 and 10. In order to elute the bacteria, the lettuce pieces were suspended into buffers and shacked for 30, 45 and 60 min. Moreover, a multiplex PCR method for the simultaneous detection of Food-borne pathogens was performed. Results: The results showed that buffer peptone water at pH 9. 5 for 45 min have high ability to elute bacteria from the lettuce surface and the bacteria can be detected using multiplex PCR. Conclusion: We developed a new rapid and efficient method for simultaneous separation of Food-borne pathogens. This method eliminates culturing stages and permits the detection and identification of target pathogens in a few hours.

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    • Cite

    APA: Copy

    Rajabzadeh, Safieh, Bahreini, Masoumeh, & Sharifmoghadam, Mohammad Reza. (2018). A rapid method for separating and concentration of food-borne pathogens using elution from ready-to-eat vegetables. IRANIAN JOURNAL OF MICROBIOLOGY, 10(6), 385-393. SID. https://sid.ir/paper/754357/en

    Vancouver: Copy

    Rajabzadeh Safieh, Bahreini Masoumeh, Sharifmoghadam Mohammad Reza. A rapid method for separating and concentration of food-borne pathogens using elution from ready-to-eat vegetables. IRANIAN JOURNAL OF MICROBIOLOGY[Internet]. 2018;10(6):385-393. Available from: https://sid.ir/paper/754357/en

    IEEE: Copy

    Safieh Rajabzadeh, Masoumeh Bahreini, and Mohammad Reza Sharifmoghadam, “A rapid method for separating and concentration of food-borne pathogens using elution from ready-to-eat vegetables,” IRANIAN JOURNAL OF MICROBIOLOGY, vol. 10, no. 6, pp. 385–393, 2018, [Online]. Available: https://sid.ir/paper/754357/en

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