Functional Surface Display of Laccase in a Phenol-Inducible Bacterial Circuit for Bioremediation Purposes

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Fathi Roudsari Mehrnoosh; BEHMANESH MEHRDAD; SALMANIAN ALI HATEF; SADEGHIZADEH MAJID; KHAJEH KHOSRO

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Journal Paper

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Journal: Iranian Biomedical Journal Year:2018 | Volume:22 | Issue:3 Page(s): 202-209

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Title

Functional Surface Display of Laccase in a Phenol-Inducible Bacterial Circuit for Bioremediation Purposes

Author(s)

Keywords

Laccase

Phenol

Bioremediation

AIDA-I

Abstract

Background: Phenolic compounds, which are produced routinely by industrial and urban activities, possess dangers to live organisms and environment. Laccases are oxidoreductase enzymes with the ability of remediating a wide variety of Phenolic compounds to more benign molecules. The purpose of the present research is surface display of a Laccase enzyme with adhesin involved in diffuse adhesion (AIDA-I) autotransporter system on the surface of Escherichia coli cells for Bioremediation of Phenolic compounds. Methods: The expression of Laccase was regulated by a Phenol-responsive promoter (a σ 54 promoter). The constitutively-expressed CapR transcription activator was able to induce Laccase expression in the presence of Phenolic compounds. Results: Western blot analysis showed the expression and correct transfer of the enzyme to the outer membrane of E. coli cells in the presence of Phenol. Activity assay confirmed the correct folding of the enzyme after translocation through the autotransporter system. HPLC analysis of residual Phenol in culture medium showed a significant reduction of Phenol concentration in the presence of cells displaying Laccase on the surface. Conclusion: Our findings confirm that autodisplay enables functional surface display of Laccase for direct substrate-enzyme availability by overcoming membrane hindrance.

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APA: Copy

Fathi Roudsari, Mehrnoosh, BEHMANESH, MEHRDAD, SALMANIAN, ALI HATEF, SADEGHIZADEH, MAJID, & KHAJEH, KHOSRO. (2018). Functional Surface Display of Laccase in a Phenol-Inducible Bacterial Circuit for Bioremediation Purposes. IRANIAN BIOMEDICAL JOURNAL, 22(3), 202-209. SID. https://sid.ir/paper/755194/en

Vancouver: Copy

Fathi Roudsari Mehrnoosh, BEHMANESH MEHRDAD, SALMANIAN ALI HATEF, SADEGHIZADEH MAJID, KHAJEH KHOSRO. Functional Surface Display of Laccase in a Phenol-Inducible Bacterial Circuit for Bioremediation Purposes. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2018;22(3):202-209. Available from: https://sid.ir/paper/755194/en

IEEE: Copy

Mehrnoosh Fathi Roudsari, MEHRDAD BEHMANESH, ALI HATEF SALMANIAN, MAJID SADEGHIZADEH, and KHOSRO KHAJEH, “Functional Surface Display of Laccase in a Phenol-Inducible Bacterial Circuit for Bioremediation Purposes,” IRANIAN BIOMEDICAL JOURNAL, vol. 22, no. 3, pp. 202–209, 2018, [Online]. Available: https://sid.ir/paper/755194/en

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