مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

171
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

161
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Protective Effect of Vitamin C on Protein Expression of Type-IV Collagen Following the Consumption of Nitrate-Containing Drinking Water in Rat Kidney

Pages

  0-0

Abstract

 Background: Evidence shows that chemical Fertilizers used for agricultural purposes have high levels of nitrate. These agricultural products consumed by Livestock are the most important sources of nitrate. Type-IV collagen, found primarily in the base membrane, is significantly vital for the performance of glomerular base membrane in the Kidney. Objectives: The present study aimed to investigate the effect of nitrate and vitamin C on the expression of type-IV collagen in rat Kidney. Methods: This empirical research was conducted on 49 Wistar Rats in Iran from 2017 to 2018. The sample size was determined using Morgan Table Samples were randomly divided into seven groups: (1) no nitrate (control), (2) nitrate at 10 mg/L, (3) nitrate at 45 mg/L, (4) nitrate at 200 mg/L, (5) nitrate (10 mg/L) + vitamin C (20 mg per 100 g of body weight), (6) nitrate (45 mg/L) + vitamin C (similar amount), and (7) nitrate (200 mg/L) + vitamin C (similar amount). After 91 days, the content of type-IV collagen in the Kidney tissue was determined using the Immunohistochemistry (IHC) protocol. The expression of type-IV collagen gene was detected by Real-Time Polymerase Chain Reaction (RT-PCR) in all groups. Results: There was no significant difference among the groups of 1-3 (4. 55 0. 51, 4. 7 0. 47, 3. 6 0. 5, P > 0. 05) in terms of type-IV collagen. However, the obtained results of group 4 indicated a significant reduction in the content of type-IV collagen (1. 25 0. 44), compared to the control group (4. 55 0. 51, P = 0. 000). In terms of vitamin C consumption, the groups of 5 (3. 45 0. 51) and 6 (3. 4  0. 5) did not differ significantly from the control group (4. 55 0. 51, P > 0. 05). Nonetheless, the severity of response to anti-type-IV collagen antibody significantly increased in group 7 (3. 55 0. 6) compared to group 4 (1. 25 0. 44, P < 0. 05). Conclusions: The investigated doses of nitrate in drinking water (up to 45 mg/L) had no significant effect on the content of type-IV collagen. On the other hand, the excessive concentrations of nitrate limited the distribution of type-IV collagen and led to potential side effects on the glomerular base membrane. Moreover, vitamin C had no significant effect on 10 and 45 mg/L doses of nitrate. Nevertheless, 200 mg/L dose of nitrate improved the destructive effects of type-IV collagen on the Kidney.

Cites

  • No record.

    • References

    Cite

    APA: Copy

    MOGHADDAM DORAFSHANI, MAHMOUD, JALALI, MAHDI, NIKRAVESH, MOHAMMAD REZA, & SOUKHTANLOO, MOHAMMAD. (2019). Protective Effect of Vitamin C on Protein Expression of Type-IV Collagen Following the Consumption of Nitrate-Containing Drinking Water in Rat Kidney. IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ), 21(7), 0-0. SID. https://sid.ir/paper/755569/en

    Vancouver: Copy

    MOGHADDAM DORAFSHANI MAHMOUD, JALALI MAHDI, NIKRAVESH MOHAMMAD REZA, SOUKHTANLOO MOHAMMAD. Protective Effect of Vitamin C on Protein Expression of Type-IV Collagen Following the Consumption of Nitrate-Containing Drinking Water in Rat Kidney. IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ)[Internet]. 2019;21(7):0-0. Available from: https://sid.ir/paper/755569/en

    IEEE: Copy

    MAHMOUD MOGHADDAM DORAFSHANI, MAHDI JALALI, MOHAMMAD REZA NIKRAVESH, and MOHAMMAD SOUKHTANLOO, “Protective Effect of Vitamin C on Protein Expression of Type-IV Collagen Following the Consumption of Nitrate-Containing Drinking Water in Rat Kidney,” IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ), vol. 21, no. 7, pp. 0–0, 2019, [Online]. Available: https://sid.ir/paper/755569/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button