An Effective Concentration of 5-Aza-CdR to Induce Cell Death and Apoptosis in Human Pancreatic Cancer Cell Line through Reactivating RASSF1A and Up-Regulation of Bax Genes

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

NIKBAKHT DASTJERDI MEHDI; AZARNEZHAD ASAAD; HASHEMIBENI BATOOL; SALEHI MANSOUR; KAZEMI MOHAMMAD; BABAZADEH ZAHRA

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS) Year:2018 | Volume:43 | Issue:5 Page(s): 533-540

Download Full-Text

View:

116

Download:

Cites:

Information Journal Paper

Title

An Effective Concentration of 5-Aza-CdR to Induce Cell Death and Apoptosis in Human Pancreatic Cancer Cell Line through Reactivating RASSF1A and Up-Regulation of Bax Genes

Author(s)

Keywords

Decitabine

Pancreatic Neoplasms

DNA modification methylases

Methylation

Abstract

Background: Promoter hyper-Methylation of tumor suppressor genes is a common event that occurs in cancer. As Methylation is a reversible modification, agents capable of reversing an abnormal Methylation status should help to combat cancer. 5-Aza-CdR is a DNA methyl-transferase inhibitor. The present study aimed to evaluate the effect of 5-Aza-CdR on the proliferation of human pancreatic cancer cell line (PANC-1) and the expression of RASSF1A and Bax genes. Methods: PANC-1 cells were cultured and treated with 5 and 10 μ M/L of 5-Aza-CdR for 24, 48, 72, and 96 hours and the percentages of cell viability and apoptosis were measured by MTT and flow cytometry. RASSF1A gene promoter Methylation was assessed by methyl-specific primer-PCR (MSP-PCR) and the expression of RASSF1A and Bax genes was measured using quantitative real-time PCR (qPCR). All quantitative data are presented as mean± SD (standard deviation). The one-way analysis of variance (ANOVA) with the LSD post hoc test was performed for statistical analysis using the SPSS software package, version 16. 0. Results: 3-[4, 5-dimethythiaziazol-2yl]-2, 5-diphenyl tetrazoliumbr omide (MTT) assay revealed that 5-Aza-CdR significantly inhibit the growth and proliferation of PANC-1. The flow cytometry results showed over 40% and 70% of early and late apoptotic cells after treatment with 5 and 10 μ m/L of 5-Aza-CdR, respectively. MSPPCR data indicated that the treatment of cells with 10 μ m/L 5-Aza-CdR resulted in partial deMethylation of RASSF1A gene promoter. qPCR results showed significant re-expression of RASSF1A and up-regulation of Bax genes after 96 hours treatment of cells with 10 μ m/L 5-Aza-CdR versus control cells (P<0. 01). Conclusion: The result demonstrated that 5 and 10 μ M of 5-Aza-CdR induce cell death and apoptosis by epigenetic reactivation of RASSF1A and up-regulation of Bax genes.

Cites

No record.

References

No record.

Cite

APA: Copy

NIKBAKHT DASTJERDI, MEHDI, AZARNEZHAD, ASAAD, HASHEMIBENI, BATOOL, SALEHI, MANSOUR, KAZEMI, MOHAMMAD, & BABAZADEH, ZAHRA. (2018). An Effective Concentration of 5-Aza-CdR to Induce Cell Death and Apoptosis in Human Pancreatic Cancer Cell Line through Reactivating RASSF1A and Up-Regulation of Bax Genes. IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS), 43(5), 533-540. SID. https://sid.ir/paper/755965/en

Vancouver: Copy

NIKBAKHT DASTJERDI MEHDI, AZARNEZHAD ASAAD, HASHEMIBENI BATOOL, SALEHI MANSOUR, KAZEMI MOHAMMAD, BABAZADEH ZAHRA. An Effective Concentration of 5-Aza-CdR to Induce Cell Death and Apoptosis in Human Pancreatic Cancer Cell Line through Reactivating RASSF1A and Up-Regulation of Bax Genes. IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS)[Internet]. 2018;43(5):533-540. Available from: https://sid.ir/paper/755965/en

IEEE: Copy

MEHDI NIKBAKHT DASTJERDI, ASAAD AZARNEZHAD, BATOOL HASHEMIBENI, MANSOUR SALEHI, MOHAMMAD KAZEMI, and ZAHRA BABAZADEH, “An Effective Concentration of 5-Aza-CdR to Induce Cell Death and Apoptosis in Human Pancreatic Cancer Cell Line through Reactivating RASSF1A and Up-Regulation of Bax Genes,” IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS), vol. 43, no. 5, pp. 533–540, 2018, [Online]. Available: https://sid.ir/paper/755965/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops