Estrogen stimulates adenosine receptor expression subtypes in human breast cancer MCF-7 cell line

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MOHAMADI AZAM; AGHAEI MAHMOUD; PANJEHPOUR MOJTABA

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Journal: RESEARCH IN PHARMACEUTICAL SCIENCES (RPS) Year:2018 | Volume:13 | Issue:1 Page(s): 57-64

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Title

Estrogen stimulates adenosine receptor expression subtypes in human breast cancer MCF-7 cell line

Author(s)

MOHAMADI AZAM | AGHAEI MAHMOUD | PANJEHPOUR MOJTABA | Issue Writer Certificate

Keywords

Breast cancer

MCF-7 cells

17β

Estradiol

Estrogen

Adenosine receptors

Abstract

Estrogen is a steroid hormone that plays a key role in the development and regulation of reproductive system. It has been shown that Estrogen is related to Breast cancer development through binding to its receptors. In order to uncover the Estrogen effects on adenosine receptor expression, Estrogen-positive MCF-7 cells were used to treat with agonist and antagonist of Estrogen and then the mRNA expression of adenosine receptor subtypes were evaluated. Estrogen-positive MCF-7 cells were treated with various concentrations of 17β; Estradiol (E2) as an Estrogen agonist, and ICI 182, 780 as an Estrogen antagonist. The gene expression of adenosine receptor subtypes were detected by real time RT-PCR. The results of MTT assay showed that E2 increased cell viability in a dose dependent manner. The expression pattern of all adenosine receptor subtypes are as follow; A2b > A1 > A2a > A3 in untreated MCF-7 cells. Obtained results showed that E2 incubation at 0. 001-0. 01 μ M led to up-regulation of A1ARs, A2aARs and A3ARs dose dependently. E2 at 0. 001 μ M also had no significant effect on A2bARs expression but, at higher doses induced a considerable decrease in mRNA A2bARs expression. Treatment with antagonist confirmed that up-regulation of these receptors is mediated by Estrogen receptor. Taken together, our results indicate that treatment of MCF-7 cells with E2 led to up-regulation of Adenosine receptors. However, these effects were partially restored by treatment with antagonist suggesting that such effects are mediated by Estrogen receptors.

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APA: Copy

MOHAMADI, AZAM, AGHAEI, MAHMOUD, & PANJEHPOUR, MOJTABA. (2018). Estrogen stimulates adenosine receptor expression subtypes in human breast cancer MCF-7 cell line. RESEARCH IN PHARMACEUTICAL SCIENCES (RPS), 13(1), 57-64. SID. https://sid.ir/paper/756102/en

Vancouver: Copy

MOHAMADI AZAM, AGHAEI MAHMOUD, PANJEHPOUR MOJTABA. Estrogen stimulates adenosine receptor expression subtypes in human breast cancer MCF-7 cell line. RESEARCH IN PHARMACEUTICAL SCIENCES (RPS)[Internet]. 2018;13(1):57-64. Available from: https://sid.ir/paper/756102/en

IEEE: Copy

AZAM MOHAMADI, MAHMOUD AGHAEI, and MOJTABA PANJEHPOUR, “Estrogen stimulates adenosine receptor expression subtypes in human breast cancer MCF-7 cell line,” RESEARCH IN PHARMACEUTICAL SCIENCES (RPS), vol. 13, no. 1, pp. 57–64, 2018, [Online]. Available: https://sid.ir/paper/756102/en

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