مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

Lentiviral vector-mediated transduction of adult neural stem/ progenitor cells isolated from the temporal tissues of epileptic patients

Pages

  354-361

Abstract

 Objective(s): Neural stem/progenitor cells (NS/PCs) hold a great potential for delivery of therapeutic agents into the injured regions of the brain. Efficient gene delivery using NS/PCs may correct a genetic defect, produce therapeutic proteins or neurotransmitters, and modulate enzyme activation. Here, we investigated the efficiency of a recombinant Lentivirus vector expressing green fluorescent protein (GFP) for genetic engineering of human NS/PCs obtained during brain surgery on patients with medically intractable epilepsy. Materials and Methods: NS/PCs were isolated from human epileptic neocortical tissues. Three plasmids (pCDH, psPAX2, pMD2. G) were used to make the virus. To produce the recombinant viruses, vectors were transmitted simultaneously into HEk-293T cells. The lentiviral particles were then used to transduce human NS/PCs. Results: Our in vitro study revealed that Lentivirus vector expressing GFP efficiently transduced about 80% of human NS/PCs. The expression of GFP was assessed as early as 3 days following exposure and remained persistent for at least 4 weeks. Conclusion: Lentiviral vectors can mediate stable, long-term expression of GFP in human NS/PCs obtained from epileptic neocortical tissues. This suggests lentiviral vectors as a potential useful tool in human NS/PCs-based gene therapy for neurological disorders, such as epilepsy.

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    • Cite

    APA: Copy

    ABDOLAHI, SARA, Khodakaram Tafti, Azizollah, ALIGHOLI, HADI, ZIAEI, SAEID, KHALEGHI GHADIRI, MARYAM, STUMMER, WALTER, & GORJI, ALI. (2020). Lentiviral vector-mediated transduction of adult neural stem/ progenitor cells isolated from the temporal tissues of epileptic patients. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 23(3), 354-361. SID. https://sid.ir/paper/756153/en

    Vancouver: Copy

    ABDOLAHI SARA, Khodakaram Tafti Azizollah, ALIGHOLI HADI, ZIAEI SAEID, KHALEGHI GHADIRI MARYAM, STUMMER WALTER, GORJI ALI. Lentiviral vector-mediated transduction of adult neural stem/ progenitor cells isolated from the temporal tissues of epileptic patients. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2020;23(3):354-361. Available from: https://sid.ir/paper/756153/en

    IEEE: Copy

    SARA ABDOLAHI, Azizollah Khodakaram Tafti, HADI ALIGHOLI, SAEID ZIAEI, MARYAM KHALEGHI GHADIRI, WALTER STUMMER, and ALI GORJI, “Lentiviral vector-mediated transduction of adult neural stem/ progenitor cells isolated from the temporal tissues of epileptic patients,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 23, no. 3, pp. 354–361, 2020, [Online]. Available: https://sid.ir/paper/756153/en

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