Expression Optimization of Anti-CD22 scFv-Apoptin Fusion Protein Using Experimental Design Methodology

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Agha Amiri Solmaz; Zarei Najmeh; ENAYATI SOMAYEH; AZIZI MOHAMMAD; KHALAJ VAHID; SHAHHOSSEINI SORAYA

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Journal: Iranian Biomedical Journal Year:2018 | Volume:22 | Issue:1 Page(s): 66-69

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Title

Expression Optimization of Anti-CD22 scFv-Apoptin Fusion Protein Using Experimental Design Methodology

Author(s)

Keywords

Recombinant protein

Single-chain antibodies

Fusion proteins

E.coli

Abstract

Background: Design of experiments is a rapid and cost-effective approach for optimization of Recombinant protein production process. In our previous study, we generated a potent dual-acting fusion protein, anti-CD22 scFv-apoptin, to target B-cell malignant cell lines. In the present investigation, we report the effect of different variables on the expression levels of this fusion protein. Methods: Four variables (cell optical density at induction, IPTG concentration, induction temperature, and induction time) were tested using experimental design. Results: Our findings demonstrated that among the examined variables, only the induction time had a significant positive effect on the protein expression yield. Conclusion: Experimental design was successfully applied in this study. The optimized condition obtained in the current study can be applied in future commercial production of this novel fusion protein.

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APA: Copy

Agha Amiri, Solmaz, Zarei, Najmeh, ENAYATI, SOMAYEH, AZIZI, MOHAMMAD, KHALAJ, VAHID, & SHAHHOSSEINI, SORAYA. (2018). Expression Optimization of Anti-CD22 scFv-Apoptin Fusion Protein Using Experimental Design Methodology. IRANIAN BIOMEDICAL JOURNAL, 22(1), 66-69. SID. https://sid.ir/paper/756331/en

Vancouver: Copy

Agha Amiri Solmaz, Zarei Najmeh, ENAYATI SOMAYEH, AZIZI MOHAMMAD, KHALAJ VAHID, SHAHHOSSEINI SORAYA. Expression Optimization of Anti-CD22 scFv-Apoptin Fusion Protein Using Experimental Design Methodology. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2018;22(1):66-69. Available from: https://sid.ir/paper/756331/en

IEEE: Copy

Solmaz Agha Amiri, Najmeh Zarei, SOMAYEH ENAYATI, MOHAMMAD AZIZI, VAHID KHALAJ, and SORAYA SHAHHOSSEINI, “Expression Optimization of Anti-CD22 scFv-Apoptin Fusion Protein Using Experimental Design Methodology,” IRANIAN BIOMEDICAL JOURNAL, vol. 22, no. 1, pp. 66–69, 2018, [Online]. Available: https://sid.ir/paper/756331/en

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