Preconditioning of Mesenchymal Stem Cells with Non-Toxic Concentration of Hydrogen Peroxide Against Oxidative Stress Induced Cell Death: The Role of Hypoxia-Inducible Factor-1

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NOURI FATEMEH; NEMATOLLAHI MAHANI SEYED NOUREDDIN; SHARIFI ALI MOHAMMAD

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Journal: Advanced Pharmaceutical Bulletin Year:2019 | Volume:9 | Issue:1 Page(s): 76-83

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Title

Preconditioning of Mesenchymal Stem Cells with Non-Toxic Concentration of Hydrogen Peroxide Against Oxidative Stress Induced Cell Death: The Role of Hypoxia-Inducible Factor-1

Author(s)

NOURI FATEMEH | NEMATOLLAHI MAHANI SEYED NOUREDDIN | SHARIFI ALI MOHAMMAD | Issue Writer Certificate

Keywords

Wharton’s jelly derived mesenchymal stem cells

Preconditioning

Hydrogen peroxide

Serum deprivation

HIF-1α

Oxidative stress

Abstract

Purpose: To investigate the protective effect of Preconditioning with non-toxic dose of Hydrogen peroxide (H2O2) as a possible cell signaling molecule, against cell death induced by toxic concentration of H2O2 or by Serum deprivation in human Wharton’ s jelly-derived mesenchymal stem cells (HWJ-MSCs) and underlying mechanisms. Methods: HWJ-MSCs were isolated and identified using flow cytometry. After finding non-toxic concentration of H2O2, cells Preconditioning was performed by H2O2 (20 μ M) for 12 h and cell tolerance against Serum deprivation or toxic levels of H2O2 was assayed by MTT test. Effect of Preconditioning on mRNA and protein expression of Akt-1, Bcl-2 and Bax were examined using reverse transcription polymerase chain reaction (RT-PCR) and western blotting respectively. Role of hypoxia-inducible factor (HIF)-1α was explored in presence HIF-1α inhibitor. Results: Preconditioning with 20 μ M H2O2 for 12 h was non-toxic and decreased cell death induced by Oxidative stress and Serum deprivation in MSC cultures. However, the increased tolerance reversed in the presence of inhibitor of HIF-1α . By regards to RT-PCR and western blotting data, although expression of Akt-1, Bcl-2 and Bax was not change considerably but phosphorylated Akt-1 (pAkt-1) was up regulated after treatment with 20 μ M H2O2 compared to control group. Moreover after exposure to 100 μ M H2O2, western blotting analysis showed that cell pretreatment with 20 μ M H2O2, decremented Bax/Bcl2 ratio and up-regulated HIF-1α and pAkt-1 compared to the control group. Conclusion: Increased tolerance of H2O2-pretreated cells led to the suggestion that transplantation of H2O2 preconditioned MSCs may improve therapeutic potential of stem cells in cell therapy procedures.

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APA: Copy

NOURI, FATEMEH, NEMATOLLAHI MAHANI, SEYED NOUREDDIN, & SHARIFI, ALI MOHAMMAD. (2019). Preconditioning of Mesenchymal Stem Cells with Non-Toxic Concentration of Hydrogen Peroxide Against Oxidative Stress Induced Cell Death: The Role of Hypoxia-Inducible Factor-1. ADVANCED PHARMACEUTICAL BULLETIN, 9(1), 76-83. SID. https://sid.ir/paper/756651/en

Vancouver: Copy

NOURI FATEMEH, NEMATOLLAHI MAHANI SEYED NOUREDDIN, SHARIFI ALI MOHAMMAD. Preconditioning of Mesenchymal Stem Cells with Non-Toxic Concentration of Hydrogen Peroxide Against Oxidative Stress Induced Cell Death: The Role of Hypoxia-Inducible Factor-1. ADVANCED PHARMACEUTICAL BULLETIN[Internet]. 2019;9(1):76-83. Available from: https://sid.ir/paper/756651/en

IEEE: Copy

FATEMEH NOURI, SEYED NOUREDDIN NEMATOLLAHI MAHANI, and ALI MOHAMMAD SHARIFI, “Preconditioning of Mesenchymal Stem Cells with Non-Toxic Concentration of Hydrogen Peroxide Against Oxidative Stress Induced Cell Death: The Role of Hypoxia-Inducible Factor-1,” ADVANCED PHARMACEUTICAL BULLETIN, vol. 9, no. 1, pp. 76–83, 2019, [Online]. Available: https://sid.ir/paper/756651/en

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