The Frequency of PAI, aer and traT Genes in Escherichia coli Commensal and Urinary Pathogenic E. coli Isolates in Shahrekord and the Relationship Between the Two Groups by Multiplex PCR

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Rezaei Mohamad Hossein; Kakian Farshad; GHOLIPOUR ABOLFAZL; ZAMANZAD BEHNAM

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Journal: Jundishapur Journal of Microbiology Year:2020 | Volume:13 | Issue:6 Page(s): 0-0

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Title

The Frequency of PAI, aer and traT Genes in Escherichia coli Commensal and Urinary Pathogenic E. coli Isolates in Shahrekord and the Relationship Between the Two Groups by Multiplex PCR

Author(s)

Rezaei Mohamad Hossein | Kakian Farshad | GHOLIPOUR ABOLFAZL | ZAMANZAD BEHNAM | Issue Writer Certificate

Keywords

Escherichia coli

Urinary Tract Infection

Antibiotic Resistance

Virulence Genes

Multiplex Polymerase Chain Reaction

Abstract

Background: Nosocomial infections are acquired during hospital treatment or in a hospitalenvironment. Onesuch infecting agent is uropathogenic Escherichia coli and many Virulence Genes enable it to become pathogenic, thereby causing damage to the host. Objectives: This study aimed to identify aer, traT, and PAI genes in E. coli isolates collected from fecal and Urinary Tract Infection (UTI) specimens and determine the relationship between them in both populations studied in a center in Iran by Multiplex Polymerase Chain Reaction (PCR) assay. Methods: Seventy-five isolates of E. coli from the urine of inpatients and 75 isolates from commensal fecal without UTI and diarrhea were collected. The E. coli bacteria were detected and isolated, using biochemical techniques and supplementary tests in the Microbiology Laboratory of Shahrekord University of Medical Sciences. Antibiotic susceptibility pattern for 14 antibiotics was done utilizing the disc diffusion method. The existence of aer, traT, and PAI Virulence Genes among all isolates was investigated by multiplex PCR. Results: Among the urinary pathogenic E. coli isolates, the highest Antibiotic Resistance was observed in cefazolin, ampicillin, and cotrimoxazole antibiotics. The prevalence rates of aer, traT, andPAI genes in the fecal isolates were 92%, 90. 6%, and46. 6%, respectively. Further, their prevalence rates in urine isolates were 96%, 97. 3%, and 41. 3%, in that order. Conclusions: The presence of the high frequency of pathogenic islands (PAIs), especially in fecal samples, is important because these genes are easily transmitted and convert a commensal bacterium into a pathogen. Because only the genome of pathogenic bacteria has been unwrapped, little attention has been paid to PAIs in commensal bacteria.

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APA: Copy

Rezaei, Mohamad Hossein, Kakian, Farshad, GHOLIPOUR, ABOLFAZL, & ZAMANZAD, BEHNAM. (2020). The Frequency of PAI, aer and traT Genes in Escherichia coli Commensal and Urinary Pathogenic E. coli Isolates in Shahrekord and the Relationship Between the Two Groups by Multiplex PCR. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 13(6), 0-0. SID. https://sid.ir/paper/757699/en

Vancouver: Copy

Rezaei Mohamad Hossein, Kakian Farshad, GHOLIPOUR ABOLFAZL, ZAMANZAD BEHNAM. The Frequency of PAI, aer and traT Genes in Escherichia coli Commensal and Urinary Pathogenic E. coli Isolates in Shahrekord and the Relationship Between the Two Groups by Multiplex PCR. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2020;13(6):0-0. Available from: https://sid.ir/paper/757699/en

IEEE: Copy

Mohamad Hossein Rezaei, Farshad Kakian, ABOLFAZL GHOLIPOUR, and BEHNAM ZAMANZAD, “The Frequency of PAI, aer and traT Genes in Escherichia coli Commensal and Urinary Pathogenic E. coli Isolates in Shahrekord and the Relationship Between the Two Groups by Multiplex PCR,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 13, no. 6, pp. 0–0, 2020, [Online]. Available: https://sid.ir/paper/757699/en

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