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Information Journal Paper

Title

Biochemical Characterization of Recombinant Thermostable Cohnella sp. A01 β-Glucanase

Pages

  345-354

Abstract

 Background: Typically, non-cellulytic glucanase, including fungi and yeast cell wall hydrolyzing enzymes, are released by some symbiotic fungi and plants during the mycoparasitic fungi attack on plants. These enzymes are known as the defense mechanisms of plants. This study intends to investigate the biochemical properties of β-1, 6-glucanase (bg16M) from native thermophilic bacteria, Cohnella A01. Methods: bg16M gene was cloned and expressed in E. coli BL21 (DE3). The enzyme was purified utilizing Ni-NTA nikcle sepharose column. Pustulan and Laminarin were selected as substrates in enzyme assay. The purified bg16M enzyme was treated with different pH, temperature, metal ions, and detergents. Results: The expressed protein, including 639 amino acids, showed a high similarity with the hydrolytic glycosylated family 30. The molecular weight of enzyme was 64 kDa, and purification yield was 46%. The bg16M demonstrated activity as 4. 83 U/ml on Laminarin and 2. 88 U/ml on Pustulan. The optimum pH and temperature of the enzyme were 8 and 50 ° C, respectively. The enzyme had an appropriate stability at high temperatures and in the pH range of 7 to 9, showing acceptable stability, while it did not lose enzymatic activity completely at acidic or basic pH. None of the studied metal ions and chemical compounds was the activator of bg16M, and urea, SDS, and copper acted as enzyme inhibitors. Conclusion: Biochemical characterization of this enzyme revealed that bg16M can be applied in beverage industries and medical sectors because of its high activity, as well as thermal and alkaline stability.

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    APA: Copy

    AMINZADEH, SAEED, Heidari, Farid, MASHHADI AKBAR BOOJAR, MASOUD, & KARKHANE, ALI ASGHAR. (2018). Biochemical Characterization of Recombinant Thermostable Cohnella sp. A01 β-Glucanase. IRANIAN BIOMEDICAL JOURNAL, 22(5), 345-354. SID. https://sid.ir/paper/758264/en

    Vancouver: Copy

    AMINZADEH SAEED, Heidari Farid, MASHHADI AKBAR BOOJAR MASOUD, KARKHANE ALI ASGHAR. Biochemical Characterization of Recombinant Thermostable Cohnella sp. A01 β-Glucanase. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2018;22(5):345-354. Available from: https://sid.ir/paper/758264/en

    IEEE: Copy

    SAEED AMINZADEH, Farid Heidari, MASOUD MASHHADI AKBAR BOOJAR, and ALI ASGHAR KARKHANE, “Biochemical Characterization of Recombinant Thermostable Cohnella sp. A01 β-Glucanase,” IRANIAN BIOMEDICAL JOURNAL, vol. 22, no. 5, pp. 345–354, 2018, [Online]. Available: https://sid.ir/paper/758264/en

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