The Inhibitory Effect of Hsa-miR-330 Replacement on the Proliferation and Migration of Breast Cancer MCF-7 Cells

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hosseinzadeh Elham; MANSOORI BEHZAD; MOHAMMADI ALI; KHAZE VAHID; REZAZADEH MARYAM; BARADARAN BEHZAD

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Journal: International Journal of Women's Health and Reproduction Sciences Year:2019 | Volume:7 | Issue:3 Page(s): 360-365

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Title

The Inhibitory Effect of Hsa-miR-330 Replacement on the Proliferation and Migration of Breast Cancer MCF-7 Cells

Author(s)

Keywords

Breast cancer

E2F1

Hsa-miR-330

Cancer therapy

Abstract

Objectives: miRNAs comprise a group of master gene expression regulators, exerting their effects after transcription through targeting specific mRNAs, hence, influencing cellular processes. A considerable number of miRNAs are known to affect cell proliferation and migration in Breast cancer, one of which is Hsa-miR-330, a key player in various types of cancers. The purpose of this study was to evaluate the anti-proliferative and anti-migrative effects of Hsa-miR-330 on MCF-7 cell line. Materials and Methods: MCF-7 cells were transfected with pCMV-miR-330 vector and cell selection was performed in media containing Geneticin (G418). Subsequently, MTT assay was performed to evaluate the effect of Hsa-miR-330 on proliferation and scratch wound healing assay was employed to evaluate cellular migration. Finally, using real-time PCR, the expression of Hsa-miR-330 as well as the repressive impact on the expression of E2F was investigated. Results: Upon confirmation of Hsa-miR-330 induction in MCF-7 cells via GFP channel imaging system, miR-330 expression was demonstrated to be increased 10 folds in stable cells. The results of MTT and wound-healing assays demonstrated an inhibitory role for Hsa-miR-330 in proliferation and migration of stable Hsa-miR-330-transfected MCF-7 cancer cells compared to controls. In addition, after transfecting cells with Hsa-miR-330, E2F1 was down-regulated in comparison with controls. Conclusions: Based on the results of the current study, we suggest a potential inhibitory role for hsa-miRNA-330 in cell proliferation as well as cell migration in Breast cancer by targeting E2F1 mRNA. Additionally, a therapeutic role can be suggested for Hsa-miR-330 in terms of target therapy for Breast cancer.

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APA: Copy

hosseinzadeh, Elham, MANSOORI, BEHZAD, MOHAMMADI, ALI, KHAZE, VAHID, REZAZADEH, MARYAM, & BARADARAN, BEHZAD. (2019). The Inhibitory Effect of Hsa-miR-330 Replacement on the Proliferation and Migration of Breast Cancer MCF-7 Cells. INTERNATIONAL JOURNAL OF WOMEN’S HEALTH AND REPRODUCTION SCIENCES, 7(3), 360-365. SID. https://sid.ir/paper/760160/en

Vancouver: Copy

hosseinzadeh Elham, MANSOORI BEHZAD, MOHAMMADI ALI, KHAZE VAHID, REZAZADEH MARYAM, BARADARAN BEHZAD. The Inhibitory Effect of Hsa-miR-330 Replacement on the Proliferation and Migration of Breast Cancer MCF-7 Cells. INTERNATIONAL JOURNAL OF WOMEN’S HEALTH AND REPRODUCTION SCIENCES[Internet]. 2019;7(3):360-365. Available from: https://sid.ir/paper/760160/en

IEEE: Copy

Elham hosseinzadeh, BEHZAD MANSOORI, ALI MOHAMMADI, VAHID KHAZE, MARYAM REZAZADEH, and BEHZAD BARADARAN, “The Inhibitory Effect of Hsa-miR-330 Replacement on the Proliferation and Migration of Breast Cancer MCF-7 Cells,” INTERNATIONAL JOURNAL OF WOMEN’S HEALTH AND REPRODUCTION SCIENCES, vol. 7, no. 3, pp. 360–365, 2019, [Online]. Available: https://sid.ir/paper/760160/en

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