LIVE TRANSPLANTATION IN CHILDREN ANTIBODY PRODUCTION AGAINST ِE. COLI POLYPEPTIDE (ECP) BY DOT BLOTTING AND ELISA METHODS FOR ECP ASSAY IN RECOMBINANT DRUG AND PATIENTS' SERA

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AHANGARI GHASEM; AKRAMI H.; SANATI M.H.; RABANI ALI

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Journal: Iranian Journal of Pediatrics Year:2003 | Volume:13 | Issue:1 Page(s): 3-10

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Title

LIVE TRANSPLANTATION IN CHILDREN ANTIBODY PRODUCTION AGAINST ِE. COLI POLYPEPTIDE (ECP) BY DOT BLOTTING AND ELISA METHODS FOR ECP ASSAY IN RECOMBINANT DRUG AND PATIENTS' SERA

Author(s)

AHANGARI GHASEM | AKRAMI H. | SANATI M.H. | RABANI ALI | Issue Writer Certificate

Keywords

POLYPEPTIDESQ4

E. COLIQ4

RECOMBINANT DRUGSQ4

IMMUNOGENECITYQ4

DOT BLOTTINGQ4

Abstract

Recombinant DNA technology made it possible to produce biological drugs and pharmaceutical companies produced recombinant human drugs. There have been reports on the formation of antibodies against residual host cell protein. For this reason, it is important to check the patients sera for antibodies which could possibly be induced by recombinant drug it self and/or by contaminating proteins of the host cell. The present report refers to the obtainment of polyspecific antisera directed against Escherichia coli host strain used to produce recombinant human growth hormone. The antisera were obtained by the cascade immunization method. The animals (n=3 rabbits) were initially immunized with E. COLI strain. The analysis of the antisera by DOT BLOTTING method showed a progressive recognition of the cascade method. These antisera were also used by ELISA methods. Results showed antibodies rising against E. COLI polypeptide antigen.

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APA: Copy

AHANGARI, GHASEM, AKRAMI, H., SANATI, M.H., & RABANI, ALI. (2003). LIVE TRANSPLANTATION IN CHILDREN ANTIBODY PRODUCTION AGAINST ِE. COLI POLYPEPTIDE (ECP) BY DOT BLOTTING AND ELISA METHODS FOR ECP ASSAY IN RECOMBINANT DRUG AND PATIENTS' SERA. IRANIAN JOURNAL OF PEDIATRICS, 13(1), 3-10. SID. https://sid.ir/paper/76068/en

Vancouver: Copy

AHANGARI GHASEM, AKRAMI H., SANATI M.H., RABANI ALI. LIVE TRANSPLANTATION IN CHILDREN ANTIBODY PRODUCTION AGAINST ِE. COLI POLYPEPTIDE (ECP) BY DOT BLOTTING AND ELISA METHODS FOR ECP ASSAY IN RECOMBINANT DRUG AND PATIENTS' SERA. IRANIAN JOURNAL OF PEDIATRICS[Internet]. 2003;13(1):3-10. Available from: https://sid.ir/paper/76068/en

IEEE: Copy

GHASEM AHANGARI, H. AKRAMI, M.H. SANATI, and ALI RABANI, “LIVE TRANSPLANTATION IN CHILDREN ANTIBODY PRODUCTION AGAINST ِE. COLI POLYPEPTIDE (ECP) BY DOT BLOTTING AND ELISA METHODS FOR ECP ASSAY IN RECOMBINANT DRUG AND PATIENTS' SERA,” IRANIAN JOURNAL OF PEDIATRICS, vol. 13, no. 1, pp. 3–10, 2003, [Online]. Available: https://sid.ir/paper/76068/en

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