مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Cites:

1

Information Journal Paper

Title

Validation of Total RNA Spin-column and Non-column Extraction Methods for microRNA Expression Analyses in Formalin-fixed Paraffin-Embedded Liver Samples

Pages

  346-349

Abstract

 Objectives: MicroRNAs are small (19– 23nt) noncoding RNAs that adjust gene expression post-transcriptionally. In addition, microRNA (miRNA) profile modification is revealed in various abnormalities including cancer. Formalin-fixed paraffinembedded (FFPE) material archives are valued for searching human diseases. Therefore, the present study aimed to investigate FFPE samples for miRNA expression, as an alternative analyte for gene expression with the growing therapeutic and diagnostic potential. Further, the validity of RNA isolation kits was evaluated in order to identify a preferred method. Materials and Methods: The two extracted methods were qualitatively and quantitatively assessed in order to compare the archived specimens between these methods. The total RNA and gene expression were quantified in each method as well. Results: Based on the results, the level of RNA extracted from FFPE tissues and real-time polymerase chain reaction (RT-PCR) of hsa-miR-623, hsa-miR-515-5pin miRNeasy FFPE (P value < 0. 047) was significantly higher than that in RNAX Plus (P < 0. 084). Conclusions: In general, the spin column method for miRNA expression profile had better expression compared to the noncolumn method. The results further confirmed the validity of FFPE tissues as an appropriate resource for miRNA analyses.

Cites

References

Cite

APA: Copy

ABHARI, ALIREZA, Shokouhi, Behrooz, LATIFI, ZEINAB, Nazari Soltan Ahmad, Saeed, Hoseinnejhad, Sirus, SAMADI, NASER, & NOURI, MOHAMMAD. (2019). Validation of Total RNA Spin-column and Non-column Extraction Methods for microRNA Expression Analyses in Formalin-fixed Paraffin-Embedded Liver Samples. CRESCENT JOURNAL OF MEDICAL AND BIOLOGICAL SCIENCES, 6(3), 346-349. SID. https://sid.ir/paper/761322/en

Vancouver: Copy

ABHARI ALIREZA, Shokouhi Behrooz, LATIFI ZEINAB, Nazari Soltan Ahmad Saeed, Hoseinnejhad Sirus, SAMADI NASER, NOURI MOHAMMAD. Validation of Total RNA Spin-column and Non-column Extraction Methods for microRNA Expression Analyses in Formalin-fixed Paraffin-Embedded Liver Samples. CRESCENT JOURNAL OF MEDICAL AND BIOLOGICAL SCIENCES[Internet]. 2019;6(3):346-349. Available from: https://sid.ir/paper/761322/en

IEEE: Copy

ALIREZA ABHARI, Behrooz Shokouhi, ZEINAB LATIFI, Saeed Nazari Soltan Ahmad, Sirus Hoseinnejhad, NASER SAMADI, and MOHAMMAD NOURI, “Validation of Total RNA Spin-column and Non-column Extraction Methods for microRNA Expression Analyses in Formalin-fixed Paraffin-Embedded Liver Samples,” CRESCENT JOURNAL OF MEDICAL AND BIOLOGICAL SCIENCES, vol. 6, no. 3, pp. 346–349, 2019, [Online]. Available: https://sid.ir/paper/761322/en

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