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Information Journal Paper

Title

Recombinant Expression and Purification of Human Cystatin C Biomarker in Escherichia coli for Prediagnostic Renal Disorders

Pages

  13-18

Keywords

Enzyme-Linked Immunosorbent Assay (ELISA) 
Glomerular Filtration Rate (GFR) 

Abstract

 Introduction: Chronic kidney disease is a worldwide health problem and Glomerular filtration rate (GFR) is the most frequently used criteria in the assessment of rental function. Cystatin C as a member of type 2 cystatin superfamily and cysteine-protease inhibitor is found in high concentrations in all biological fluids. This study is aimed to study Cystatin C as a potent biomarker for clinical measurement of renal disorders and other diseases. Materials and Methods: In this study, the human Cystatin C construct was analyzed by bioinformatics software. It was cloned and expressed to produce an appropriate antigen for anti-Cystatin C (anti-Cys C) obtained from mice Balb/C as a crucial point in the improvement of an enzyme-linked immunosorbent assay (ELISA) method. Serum samples were given from 32 hospitalized patients with renal failure and cardiovascular disease and non-hospitalized patients were tested by ELISA method using anti-Cys C obtained from mice Balb/C. Results: Our findings indicated 0. 36-2. 4 mg/L as the best conclusion for antigen Cystatin C in patients’ sera and 1/50 dilution for anti-Cys C obtained from mice Balb/C and showed a relationship between patients with high Creatinine and high concentration of Cystatin C. In case of five cardiovascular disease patients with normal upper limit of Creatinine we obtained Cystatin C lower than kidney failure and raising of Cystatin C in 6 patients with increased TSH were seen. Conclusions: Polyclonal anti-Cys C antibodies were obtained through the immunization of Balb/C mice can be employed as an anti-Cys C in ELISA for diagnosis of some renal dysfunction.

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    • Cite

    APA: Copy

    Abdollah, Zahra, KHODI, SAMANEH, Gheybi, Elaheh, AMANI, JAFAR, & KARAMI, ALI. (2018). Recombinant Expression and Purification of Human Cystatin C Biomarker in Escherichia coli for Prediagnostic Renal Disorders. JOURNAL OF APPLIED BIOTECHNOLOGY REPORTS, 5(1), 13-18. SID. https://sid.ir/paper/761456/en

    Vancouver: Copy

    Abdollah Zahra, KHODI SAMANEH, Gheybi Elaheh, AMANI JAFAR, KARAMI ALI. Recombinant Expression and Purification of Human Cystatin C Biomarker in Escherichia coli for Prediagnostic Renal Disorders. JOURNAL OF APPLIED BIOTECHNOLOGY REPORTS[Internet]. 2018;5(1):13-18. Available from: https://sid.ir/paper/761456/en

    IEEE: Copy

    Zahra Abdollah, SAMANEH KHODI, Elaheh Gheybi, JAFAR AMANI, and ALI KARAMI, “Recombinant Expression and Purification of Human Cystatin C Biomarker in Escherichia coli for Prediagnostic Renal Disorders,” JOURNAL OF APPLIED BIOTECHNOLOGY REPORTS, vol. 5, no. 1, pp. 13–18, 2018, [Online]. Available: https://sid.ir/paper/761456/en

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