EFFECT OF EGF ON THE DEVELOPMENT OF 16-18 CELLED MOUSE EMBRYOS AND EXPRESSION OF EGFR AFTER VITRIFICATION

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

GHORBANMEHR N.; MOVAHEDIN M.; MOULA SEYED JAVAD

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES Year:2006 | Volume:11 | Issue:1 (Serial number 39) Page(s): 60-70

Download Full-Text

Persian Verion

View:

1,387

Download:

Cites:

Information Journal Paper

Title

EFFECT OF EGF ON THE DEVELOPMENT OF 16-18 CELLED MOUSE EMBRYOS AND EXPRESSION OF EGFR AFTER VITRIFICATION

Author(s)

GHORBANMEHR N. | MOVAHEDIN M. | MOULA SEYED JAVAD | Issue Writer Certificate

Keywords

PREIMPLANTATION EMBRYOQ3

EPIDERMAL GROWTH FACTORQ3

CRYOPRESERVATIONQ3

GENE EXPRESSIONQ2

Abstract

Background and Aim: Successful development and implantation of cryo-preserved embryos depend on suitable culture medium, cytokines, growth factors and genomic expression. EPIDERMAL GROWTH FACTOR and its receptor have important roles on embryonic development and implantation. The purpose of this study was to investigate the influence of EGF on preimplantation development and to detect mRNA for the EGF receptor in vitrified mouse embryo.Materials and Methods: 8 to 16 celled mouse embryos were collected from super-ovulated NMRI mice 56 to 64 h after RCG injection and divided into 4 groups: two control groups were cultured in MEM-a (control 1) and MEM-α+EGF (10 ng/ml) as control 2. The experimental groups were vitrified immediately after collection and then cultured for 96h in MEM-a and M-α+EGF (l0 ng/ml) respectively. Expression of EGFR in hatched and hatching blastocysts was studied too. Following RT, PCR nested primers were designed to optimize the specificity.Results: The results showed that blastocyst formation and hatching rates were significantly lower in vitrified embryos and degeneration rate was significantly higher in these groups. Blastocyst formation, hatching and degeneration rates were not significantly different in control group 1 compared to control group 2 and also in expo group 1 in comparison with exp. group 2. 96 hours after culture, mRNA expression of EGFR was detected in embryos in the four groups.Conclusion: In conclusion addition of EGF (10 ng/ml) to a complex medium like (MEM-a) doesn't have any stimulatory effect on embryonic development of vitrified and non-vitrified embryos. Vitrification did not prevent EGFR expression after 96h of culturing, but it seems that addition of EGF to the medium stimulates EGFR expression in mouse blastocysts.

Cites

No record.

References

No record.

Cite

APA: Copy

GHORBANMEHR, N., MOVAHEDIN, M., & MOULA, SEYED JAVAD. (2006). EFFECT OF EGF ON THE DEVELOPMENT OF 16-18 CELLED MOUSE EMBRYOS AND EXPRESSION OF EGFR AFTER VITRIFICATION. SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES, 11(1 (Serial number 39)), 60-70. SID. https://sid.ir/paper/76278/en

Vancouver: Copy

GHORBANMEHR N., MOVAHEDIN M., MOULA SEYED JAVAD. EFFECT OF EGF ON THE DEVELOPMENT OF 16-18 CELLED MOUSE EMBRYOS AND EXPRESSION OF EGFR AFTER VITRIFICATION. SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2006;11(1 (Serial number 39)):60-70. Available from: https://sid.ir/paper/76278/en

IEEE: Copy

N. GHORBANMEHR, M. MOVAHEDIN, and SEYED JAVAD MOULA, “EFFECT OF EGF ON THE DEVELOPMENT OF 16-18 CELLED MOUSE EMBRYOS AND EXPRESSION OF EGFR AFTER VITRIFICATION,” SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES, vol. 11, no. 1 (Serial number 39), pp. 60–70, 2006, [Online]. Available: https://sid.ir/paper/76278/en

Related Journal Papers