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Information Journal Paper

Title

A Validated HPLC Method for Quantitation of Rosmarinic Acid in a Polyherbal Syrup

Pages

  5-11

Abstract

 Background and objectives: “ Monzej-e Soda” is a poly Herbal decoction which is used in Iranian traditional medicine for many diseases especially the ones with similar signs to depression. In this investigation, an HPLC method for quantitation of Rosmarinic acid as a marker of the “ Monzej-e Soda” syrup has been developed and validated. Methods: The syrup was prepared by decocting a mixture containing Lavandula angustifolia, Melissa officinalis, Echium amoenum, Cordia myxa, Glycyrrhiza glabra, Ziziphus jujuba, Foeniculum vulgare, Fumaria parviflora, Adiantum capillus-veneris and Alhagi spp. Manna. Then, an HPLC technique was designed for analysis of Rosmarinic acid in the syrup using C18 column (4. 6 × 250 mm, 5 μ m); H3PO4 0. 085%: acetonitrile as the mobile phase in gradient mode. The flow rate, column temperature, wave length, injection volume and run time were 1 mL/min, 40 ᵒ C, 330 nm, 20 μ L and 34 min, respectively. The method was validated for selectivity, linearity, precision, accuracy, LOD and LOQ. Results: By using the proposed method, Rosmarinic acid was completely separated from the other components in the syrup chromatogram with acceptable peak shape. The concentration of Rosmarinic acid in the syrup was found 47. 5 mg/100 mL. The HPLC method was valid according to selectivity, linearity (72-110 μ g/mL, r2: 0. 9995), intra-day and inter-day precisions (RSD%˂ 2), accuracy (103. 38-106. 47%), LOD 1. 6 μ g/mL and LOQ 4. 9 μ g/mL. Conclusion: This method is a simple and suitable one for quantitation of Rosmarinic acid in “ Monzej-e Soda” syrup and could be used for quality control of the syrup.

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    Cite

    APA: Copy

    Zakerin, Sara, HAJIMEHDIPOOR, HOMA, MORTAZAVI, SEYED ALIREZA, CHOOPANI, RASOOL, FAHIMI, SHIRIN, SABETKASAEI, MASOUMEH, & Tavakolifar, Fatemeh. (2020). A Validated HPLC Method for Quantitation of Rosmarinic Acid in a Polyherbal Syrup. RESEARCH JOURNAL OF PHARMACOGNOSY, 7(2), 5-11. SID. https://sid.ir/paper/765337/en

    Vancouver: Copy

    Zakerin Sara, HAJIMEHDIPOOR HOMA, MORTAZAVI SEYED ALIREZA, CHOOPANI RASOOL, FAHIMI SHIRIN, SABETKASAEI MASOUMEH, Tavakolifar Fatemeh. A Validated HPLC Method for Quantitation of Rosmarinic Acid in a Polyherbal Syrup. RESEARCH JOURNAL OF PHARMACOGNOSY[Internet]. 2020;7(2):5-11. Available from: https://sid.ir/paper/765337/en

    IEEE: Copy

    Sara Zakerin, HOMA HAJIMEHDIPOOR, SEYED ALIREZA MORTAZAVI, RASOOL CHOOPANI, SHIRIN FAHIMI, MASOUMEH SABETKASAEI, and Fatemeh Tavakolifar, “A Validated HPLC Method for Quantitation of Rosmarinic Acid in a Polyherbal Syrup,” RESEARCH JOURNAL OF PHARMACOGNOSY, vol. 7, no. 2, pp. 5–11, 2020, [Online]. Available: https://sid.ir/paper/765337/en

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