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Information Journal Paper

Title

Bioinspired Device Improves The Cardiogenic Potential of Cardiac Progenitor Cells

Pages

  129-136

Abstract

 Objective: Functional Cardiac Tissue Engineering holds promise as a candidate approach for myocardial infarction. Tissue engineering has emerged to generate functional tissue constructs and provide an alternative means to repair and regenerate damaged heart tissues. Materials and Methods: In this experimental study, we fabricated a composite polycaprolactone (PCL)/gelatine electrospun scaffold with aligned nanofibres. The electrospinning parameters and optimum proportion of the PCL/ gelatine were tested to design a scaffold with aligned and homogenized nanofibres. Using scanning electron microscopy (SEM) and mechanophysical testes, the PCL/gelatine composite scaffold with a ratio of 70: 30 was selected. In order to simulate cardiac contraction, a developed mechanical loading device (MLD) was used to apply a mechanical stress with specific frequency and tensile rate to Cardiac Progenitor Cells (CPCs) in the direction of the aligned nanofibres. Cell metabolic determination of CPCs was performed using real-time polymerase chain reaction(RT-PCR). Results: Physicochemical and mechanical characterization showed that the PCL/gelatine composite scaffold with a ratio of 70: 30 was the best sample. In vitro analysis showed that the scaffold supported active metabolism and proliferation of CPCs, and the generation of uniform cellular constructs after five days. Real-time PCR analysis revealed elevated expressions of the specific genes for synchronizing beating cells (MYH-6, TTN and CX-43) on the dynamic scaffolds compared to the control sample with a static culture system. Conclusion: Our study provides a robust platform for generation of synchronized beating cells on a nanofibre patch that can be used in Cardiac Tissue Engineering applications in the near future.

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    APA: Copy

    SHAMS, ZAHRA, Akbari, Babak, RAJABI, SARAH, & AGHDAMI, NASSER. (2021). Bioinspired Device Improves The Cardiogenic Potential of Cardiac Progenitor Cells. CELL JOURNAL (YAKHTEH), 23(1 (89)), 129-136. SID. https://sid.ir/paper/765750/en

    Vancouver: Copy

    SHAMS ZAHRA, Akbari Babak, RAJABI SARAH, AGHDAMI NASSER. Bioinspired Device Improves The Cardiogenic Potential of Cardiac Progenitor Cells. CELL JOURNAL (YAKHTEH)[Internet]. 2021;23(1 (89)):129-136. Available from: https://sid.ir/paper/765750/en

    IEEE: Copy

    ZAHRA SHAMS, Babak Akbari, SARAH RAJABI, and NASSER AGHDAMI, “Bioinspired Device Improves The Cardiogenic Potential of Cardiac Progenitor Cells,” CELL JOURNAL (YAKHTEH), vol. 23, no. 1 (89), pp. 129–136, 2021, [Online]. Available: https://sid.ir/paper/765750/en

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