Effect of benomyl and diazinon on acquired azole resistance in Aspergillus flavus and expression of mdr1 and cyp51c genes

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

AKBARI DANA MARYAM; HASHEMI SEYED JAMAL; Daei Ghazvini Roshanak; Khodavesi Sadegh; MODIRI MONA; Nazemi Ladan; Darabian Sima; REZAIE SASAN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Current Medical Mycology Year:2019 | Volume:5 | Issue:2 Page(s): 27-32

Download Full-Text

View:

128

Download:

Cites:

Information Journal Paper

Title

Effect of benomyl and diazinon on acquired azole resistance in Aspergillus flavus and expression of mdr1 and cyp51c genes

Author(s)

Keywords

Aspergillus flavus

Azole resistant

Gene expression

Pesticides

Abstract

Background and Purpose: Aspergillus flavus is an important pathogen in immunodeficient patients. Due to the abundance of this fungus in nature, fungicides are commonly used to preserve and maintain agricultural products. Long-term exposure to these Pesticides can lead to the induction of drug resistance in this fungus. Materials and Methods: For the purpose of the study, 10 strains of A. flavus ATCC 204304 were cultured in benomyl and diazinon Pesticides at the concentrations of 62. 5, 125, 250. 500, 750, 1000, 1500, 2000, and 2500 mg/L in nine steps. Morphological changes and resistance to voriconazole, itraconazole, and amphotericin B were evaluated at the end of each step. Subsequently, changes in the expression of mdr1 and cyp51C genes were studied in the strains showing drug resistance. Results: The results showed that during the nine stages of the adjacency of strains with benomyl and diazinon at different concentrations, resistance to voriconazole, itraconazole, and amphotericin B in these toxins increased by 30% and 10%, respectively. In addition, the microscopic examination of resistant strains revealed the absence of sporulation, and only mycelium was found. Macroscopically, the color of the colonies changed from green to white. Furthermore, the investigation of the expression of mdr1 and cyp51c genes in these strains showed a decrease and increase in adjacency with diazinon and benomyl, respectively. Conclusion: As the findings indicated, exposure to agricultural Pesticides can lead to the incidence of morphological changes and resistance to amphotericin B, itraconazole, and voriconazole in the sensitive species of A. flavus by altering the expression of genes involved in drug resistance.

Cites

No record.

References

No record.

Cite

APA: Copy

AKBARI DANA, MARYAM, HASHEMI, SEYED JAMAL, Daei Ghazvini, Roshanak, Khodavesi, Sadegh, MODIRI, MONA, Nazemi, Ladan, Darabian, Sima, & REZAIE, SASAN. (2019). Effect of benomyl and diazinon on acquired azole resistance in Aspergillus flavus and expression of mdr1 and cyp51c genes. CURRENT MEDICAL MYCOLOGY, 5(2), 27-32. SID. https://sid.ir/paper/767499/en

Vancouver: Copy

AKBARI DANA MARYAM, HASHEMI SEYED JAMAL, Daei Ghazvini Roshanak, Khodavesi Sadegh, MODIRI MONA, Nazemi Ladan, Darabian Sima, REZAIE SASAN. Effect of benomyl and diazinon on acquired azole resistance in Aspergillus flavus and expression of mdr1 and cyp51c genes. CURRENT MEDICAL MYCOLOGY[Internet]. 2019;5(2):27-32. Available from: https://sid.ir/paper/767499/en

IEEE: Copy

MARYAM AKBARI DANA, SEYED JAMAL HASHEMI, Roshanak Daei Ghazvini, Sadegh Khodavesi, MONA MODIRI, Ladan Nazemi, Sima Darabian, and SASAN REZAIE, “Effect of benomyl and diazinon on acquired azole resistance in Aspergillus flavus and expression of mdr1 and cyp51c genes,” CURRENT MEDICAL MYCOLOGY, vol. 5, no. 2, pp. 27–32, 2019, [Online]. Available: https://sid.ir/paper/767499/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops