Cellular apoptosis: An alternative mechanism of action for caspofungin against Candida glabrata

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Aryamloo Parisa; ASGARIAN OMRAN HOSSEIN; ASLANI NARGES; HOSSEIN NATAJ HADI; SHOKOHI TAHEREH; BADALI HAMID; NABILI MOJTABA; Abdollahi Gohar Atefeh; MOAZENI MARYAM

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Journal: Current Medical Mycology Year:2019 | Volume:5 | Issue:2 Page(s): 9-15

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Title

Cellular apoptosis: An alternative mechanism of action for caspofungin against Candida glabrata

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Keywords

Candida glabrata

Caspofungin

Flow cytometry

MCA1

NUC1

Abstract

ackground and Purpose: Although the mechanism of action for echinocandins is known, the physiological mechanisms by which these antifungal agents cause cell death via the classical apoptotic pathways are not well-defined yet. Regarding this, the present study aimed to evaluate the mechanisms of Caspofungin-induced Candida glabrata cell death. Materials and Methods: For the purpose of the study, the minimum inhibitory concentration (MIC) of Caspofungin against C. glabrata (ATCC 90030) was determined using the broth microdilution reference method (CLSI M27-A2 and M27-S4). The annexin V and propidium iodide staining was performed to determine the way through which Caspofungin acts against C. glabrata (i. e., through the induction of apoptosis and/or necrosis). Additionally, the possible effect of Caspofungin on inducing the expression of two apoptotic genes, namely MCA1 and NUC, was studied using the realtime polymerase chain reaction assay. Results: According to the obtained MIC value (0. 5 µ g/mL), C. glabrata, exposed to 0. 25, 0. 5, and 1 µ g/mL of Caspofungin, exhibited the features of late apoptosis/necrosis after 18 h of incubation. Furthermore, the use of 0. 25, 0. 5, and 1 µ g/ml Caspofungin induced apoptosis (early/late) in 14. 67%, 17. 04%, and 15. 89% of the cells, respectively. The results showed a significant difference between the percentages of early-apoptotic cells at the three concentrations (P<0. 05). In addition, the rate of necrosis was significantly greater than that of apoptosis in response to Caspofungin. Accordingly, necrosis occurred in 71. 26%, 71. 26%, and 61. 26% of the cells at the Caspofungin concentrations of 0. 25, 0. 5, and 1 µ g/mL, respectively (P<0. 05). The analysis of the data in the REST software demonstrated a significant increase in the expression of MCA1 and NUC1 genes (P<0. 05). Conclusion: As the findings of the present study indicated, Caspofungin promoted both necrosis and apoptosis of C. glabrata cells at concentrations higher than or equal to the MIC value.

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Investigating the Effect of Endurance Training and Supplementation of Persia Rosa Extract on Mitochondrial Apoptosis of Myocardium

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APA: Copy

Aryamloo, Parisa, ASGARIAN OMRAN, HOSSEIN, ASLANI, NARGES, HOSSEIN NATAJ, HADI, SHOKOHI, TAHEREH, BADALI, HAMID, NABILI, MOJTABA, Abdollahi Gohar, Atefeh, & MOAZENI, MARYAM. (2019). Cellular apoptosis: An alternative mechanism of action for caspofungin against Candida glabrata. CURRENT MEDICAL MYCOLOGY, 5(2), 9-15. SID. https://sid.ir/paper/767947/en

Vancouver: Copy

Aryamloo Parisa, ASGARIAN OMRAN HOSSEIN, ASLANI NARGES, HOSSEIN NATAJ HADI, SHOKOHI TAHEREH, BADALI HAMID, NABILI MOJTABA, Abdollahi Gohar Atefeh, MOAZENI MARYAM. Cellular apoptosis: An alternative mechanism of action for caspofungin against Candida glabrata. CURRENT MEDICAL MYCOLOGY[Internet]. 2019;5(2):9-15. Available from: https://sid.ir/paper/767947/en

IEEE: Copy

Parisa Aryamloo, HOSSEIN ASGARIAN OMRAN, NARGES ASLANI, HADI HOSSEIN NATAJ, TAHEREH SHOKOHI, HAMID BADALI, MOJTABA NABILI, Atefeh Abdollahi Gohar, and MARYAM MOAZENI, “Cellular apoptosis: An alternative mechanism of action for caspofungin against Candida glabrata,” CURRENT MEDICAL MYCOLOGY, vol. 5, no. 2, pp. 9–15, 2019, [Online]. Available: https://sid.ir/paper/767947/en

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