مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Cites:

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Information Journal Paper

Title

Detection of lead ions using an electrochemical aptasensor

Pages

  247-252

Abstract

 The aim of this work was to develop a simple, selective and sensitive Aptasensor for detection of Pb2+ based on hairpin structure of complementary strand (CS) of aptamer (Apt). Screen printed carbon electrode (SPCE) was electrodeposited by Gold nanoparticles to be used as the substrate for the immobilization of Apt-CS. Moreover, Gold nanoparticles (AuNPs) and thionine were added to increase the sensitivity of the Aptasensor. The morphology of SPCE and AuNPs modified SPCE was investigated using scanning electron microscopy (SEM). The SEM results exhibited that the AuNPs were homogeneously distributed on the surface of SPCE. The electrochemical performance of Aptasensor was evaluated by means of Cyclic voltammetry (CV) and Differential pulse voltammetry (DPV) measurements. The electrochemical results indicated that peak current due to the hairpin structure formation of CS decreased in the presence of Pb2+. However, in the absence of Pb2+, peak current was enhanced because of combination of thionine/AuNPs-CS. The designed Aptasensor showed a high selectivity toward Pb2+, wide linear range (1-40 nM), low detection limit (374 pM), acceptable reproducibility and good long-term stability.

Cites

References

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    • Cite

    APA: Copy

    Adabi, Mohsen. (2019). Detection of lead ions using an electrochemical aptasensor. NANOMEDICINE RESEARCH JOURNAL, 4(4), 247-252. SID. https://sid.ir/paper/769474/en

    Vancouver: Copy

    Adabi Mohsen. Detection of lead ions using an electrochemical aptasensor. NANOMEDICINE RESEARCH JOURNAL[Internet]. 2019;4(4):247-252. Available from: https://sid.ir/paper/769474/en

    IEEE: Copy

    Mohsen Adabi, “Detection of lead ions using an electrochemical aptasensor,” NANOMEDICINE RESEARCH JOURNAL, vol. 4, no. 4, pp. 247–252, 2019, [Online]. Available: https://sid.ir/paper/769474/en

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