Identification of clinical dermatophyte isolates obtained from Iran by matrix-assisted laser desorption/ionization time-offlight mass spectrometry

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HEDAYATI MOHAMMAD TAGHI; Ansari Saham; Ahmadi Bahram; TAGHIZADEH ARMAKI MOJTABA; SHOKOHI TAHEREH; ABASTABAR MAHDI; Er Halil; ozhak betil; ogunc Dilara; Ilkit Macit; SEYEDMOUSAVI SEYEDMOJTABA

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Journal: Current Medical Mycology Year:2019 | Volume:5 | Issue:2 Page(s): 22-26

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Title

Identification of clinical dermatophyte isolates obtained from Iran by matrix-assisted laser desorption/ionization time-offlight mass spectrometry

Author(s)

Keywords

Dermatophytes

Iran

ITS phylogeny

MALDI-TOF MS

Abstract

Background and Purpose: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is widely used to discriminate among pathogenic microorganisms in clinical laboratories. The aim of this study was to assess the utility of MALDI-TOF MS in the routine identification of clinical dermatophyte isolates obtained from various geographical regions of Iran. Materials and Methods: A total of 94 isolates, including Trichophyton interdigitale (n=44), T. rubrum (n=40), T. tonsurans (n=4), Microsporum canis (n=4), and Epidermophyton floccosum (n=1), were analyzed in this study. The identity of each isolate was determined by polymerase chani reaction amplification and sequencing of the internal transcribed spacer (ITS) region of nuclear-encoded ribosomal DNA and also MALDI-TOF MS. The obtained data by molecular approach were compared with MALDI-TOF MS. Results: The MALDI-TOF MS led to the identification of 44 (47%) isolates at the species level by generating the spectral score values of ≥ 2. 0. However, there was not sufficient agreement between the results of the molecular-based ITS identification methods and MALDI-TOF MS in the species identification of 16 (17%) isolates. The Bruker Daltonics database was also not able to identify protein spectra related to 12 isolates (13%), including T. interdigitale (n=5), T. rubrum (n=4), M. canis (n=2), and T. tonsurans (n=1). Conclusion: According to the results, the utility of MALDI-TOF MS as a routine diagnostic tool for the accurate and reliable identification of Dermatophytes can be justified whenever the protein spectra of a large set of worldwide clinical isolates are included in the commercial libraries. In addition, MALDI-TOF MS can be alternatively used to construct an in-house reference database.

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APA: Copy

HEDAYATI, MOHAMMAD TAGHI, Ansari, Saham, Ahmadi, Bahram, TAGHIZADEH ARMAKI, MOJTABA, SHOKOHI, TAHEREH, ABASTABAR, MAHDI, Er, Halil, ozhak, betil, ogunc, Dilara, & Ilkit, Macit. (2019). Identification of clinical dermatophyte isolates obtained from Iran by matrix-assisted laser desorption/ionization time-offlight mass spectrometry. CURRENT MEDICAL MYCOLOGY, 5(2), 22-26. SID. https://sid.ir/paper/774605/en

Vancouver: Copy

HEDAYATI MOHAMMAD TAGHI, Ansari Saham, Ahmadi Bahram, TAGHIZADEH ARMAKI MOJTABA, SHOKOHI TAHEREH, ABASTABAR MAHDI, Er Halil, ozhak betil, ogunc Dilara, Ilkit Macit. Identification of clinical dermatophyte isolates obtained from Iran by matrix-assisted laser desorption/ionization time-offlight mass spectrometry. CURRENT MEDICAL MYCOLOGY[Internet]. 2019;5(2):22-26. Available from: https://sid.ir/paper/774605/en

IEEE: Copy

MOHAMMAD TAGHI HEDAYATI, Saham Ansari, Bahram Ahmadi, MOJTABA TAGHIZADEH ARMAKI, TAHEREH SHOKOHI, MAHDI ABASTABAR, Halil Er, betil ozhak, Dilara ogunc, and Macit Ilkit, “Identification of clinical dermatophyte isolates obtained from Iran by matrix-assisted laser desorption/ionization time-offlight mass spectrometry,” CURRENT MEDICAL MYCOLOGY, vol. 5, no. 2, pp. 22–26, 2019, [Online]. Available: https://sid.ir/paper/774605/en

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