Juxtaposition of Mesenchymal Stem Cells with Endothelial Progenitor Cells Promoted Angiogenic Potential Inside Alginate-Gelatin Microspheres

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SABERIANPOUR SHIRIN; RAHBARGHAZI REZA; AHMADI MAHDI; NOURI MOHAMMAD; Heidarzadeh Morteza; KARIMI ABBAS; Nemati Souror

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Journal: Advanced Pharmaceutical Bulletin Year:2021 | Volume:11 | Issue:1 Page(s): 163-170

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Title

Juxtaposition of Mesenchymal Stem Cells with Endothelial Progenitor Cells Promoted Angiogenic Potential Inside Alginate-Gelatin Microspheres

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Vitronectin

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Abstract

Purpose: Here, we investigated the angiogenic potential of endothelial progenitor cells juxtaposed with mesenchymal stem cells (MSCs) inside alginate-gelatin microspheres with stromal derived factor-1α (SDF-1 α ) for 7 days. Methods: Six encapsulated groups were allocated including endothelial progenitor cells (EPCs), EPCs/SDF-1α , MSCs, MSCs/SDF-1α , EPCs+MSCs and EPCs+MSCs/SDF-1α . Cells were encapsulated with a mixture of 1% alginate and 2% gelatin hydrogel. Cell survival was examined by MTT assay. Endothelial differentiation was determined by flow cytometry and ELISA. Tubulogenesis assay and Ac-Dil-LDL uptake were used to detect functional activity. Cell migration was analyzed by Transwell insert and gelatin zymography analyses. By using real-time polymerase chain reaction (PCR), we measured the transcription of Akt and PK1. Results: We found an increase in cell viability in MSCs/SDF-1α microspheres compared to EPCs group (P < 0. 05). EPC/MSCs co-culture contributed to the increase of CD133+ cells while we found high CD31 levels in MSCs group (P < 0. 05). Juxtaposition of EPC with MSCs increased tubulogenesis compared to SDF-1a-free condition (P < 0. 001). SDF-1α had the potential to increase in AC-LDL uptake in MSCs and EPCs/MSCs groups. Cells migration and MMP-9 activities increased after treatment with SDF-1α . SDF-1α upregulated PK1 and Akt in encapsulated cells, especially in a co-culture system. Conclusion: Alginate-gelatin microspheres could alter the angiogenic potential of progenitor cells in the presence of SDF-1α.

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APA: Copy

SABERIANPOUR, SHIRIN, RAHBARGHAZI, REZA, AHMADI, MAHDI, NOURI, MOHAMMAD, Heidarzadeh, Morteza, KARIMI, ABBAS, & Nemati, Souror. (2021). Juxtaposition of Mesenchymal Stem Cells with Endothelial Progenitor Cells Promoted Angiogenic Potential Inside Alginate-Gelatin Microspheres. ADVANCED PHARMACEUTICAL BULLETIN, 11(1), 163-170. SID. https://sid.ir/paper/775338/en

Vancouver: Copy

SABERIANPOUR SHIRIN, RAHBARGHAZI REZA, AHMADI MAHDI, NOURI MOHAMMAD, Heidarzadeh Morteza, KARIMI ABBAS, Nemati Souror. Juxtaposition of Mesenchymal Stem Cells with Endothelial Progenitor Cells Promoted Angiogenic Potential Inside Alginate-Gelatin Microspheres. ADVANCED PHARMACEUTICAL BULLETIN[Internet]. 2021;11(1):163-170. Available from: https://sid.ir/paper/775338/en

IEEE: Copy

SHIRIN SABERIANPOUR, REZA RAHBARGHAZI, MAHDI AHMADI, MOHAMMAD NOURI, Morteza Heidarzadeh, ABBAS KARIMI, and Souror Nemati, “Juxtaposition of Mesenchymal Stem Cells with Endothelial Progenitor Cells Promoted Angiogenic Potential Inside Alginate-Gelatin Microspheres,” ADVANCED PHARMACEUTICAL BULLETIN, vol. 11, no. 1, pp. 163–170, 2021, [Online]. Available: https://sid.ir/paper/775338/en

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