EVALUATION OF THE GENETIC VARIATION OF NON CODING CONTROL REGION OF BK VIRUS USING NESTED-PCR SEQUENCING METHOD IN RENAL GRAFT PATIENTS

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EMAMI A.; YAGHOBI R.; MOATTARI A.; BASERI SALEHI M.; ROOZBEH J.

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Journal: Armaghane Danesh Year:2015 | Volume:20 | Issue:2 (97) Page(s): 89-102

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Title

EVALUATION OF THE GENETIC VARIATION OF NON CODING CONTROL REGION OF BK VIRUS USING NESTED-PCR SEQUENCING METHOD IN RENAL GRAFT PATIENTS

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Keywords

POLYOMAVIRUS BKQ2

RENAL GRAFTQ3

NEPHROPATHYQ2

NON CODING CONTROL REGIONQ2

Abstract

Background: POLYOMAVIRUS BK is a ubiquitous infectious agent with more than totally 80% prevalence in the world. One of the clinical importances of POLYOMAVIRUS BK is association with of kidney injury and graft rejection in kidney transplant recipients. NON CODING CONTROL REGION of the genome of this virus play a regulatory role in replication and amplification of the virus. This study was performed to design a specific and sensitive molecular technique to evaluate of the genetic variation of NON CODING CONTROL REGION of POLYOMAVIRUS BK in kidney transplant patients Materials and Methods: In this study, total of 129 urine samples were collected from RENAL GRAFT patients.Then, for the first time an in-house-nested-PCR sequencing method was optimized to evaluate of the possible genetic variation of NON CODING CONTROL REGION of POLYOMAVIRUS BK in urine samples of studied kidney transplant patients.Results: Urine samples were collected from 76 (58.9%) males and 46 (35.7%) females with the mean age 38.0±.089 years. Totally 76 (58.9%) samples were positive for POLYOMAVIRUS BK infection. The algorithm of the sequences of NON CODING CONTROL REGION was identified in polyomavirus infected urine samples.Conclusion: Sequence analysis of the NON CODING CONTROL REGIONs of POLYOMAVIRUS BK genome among urine samples showed a widespread rearranged genetic format in this region of viral genome among kidney transplant patients. Evaluation of these sequences showed a defined and different genetic algorithm form standard Archetype strains among studied patients.

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APA: Copy

EMAMI, A., YAGHOBI, R., MOATTARI, A., BASERI SALEHI, M., & ROOZBEH, J.. (2015). EVALUATION OF THE GENETIC VARIATION OF NON CODING CONTROL REGION OF BK VIRUS USING NESTED-PCR SEQUENCING METHOD IN RENAL GRAFT PATIENTS. ARMAGHAN DANESH, 20(2 (97)), 89-102. SID. https://sid.ir/paper/78097/en

Vancouver: Copy

EMAMI A., YAGHOBI R., MOATTARI A., BASERI SALEHI M., ROOZBEH J.. EVALUATION OF THE GENETIC VARIATION OF NON CODING CONTROL REGION OF BK VIRUS USING NESTED-PCR SEQUENCING METHOD IN RENAL GRAFT PATIENTS. ARMAGHAN DANESH[Internet]. 2015;20(2 (97)):89-102. Available from: https://sid.ir/paper/78097/en

IEEE: Copy

A. EMAMI, R. YAGHOBI, A. MOATTARI, M. BASERI SALEHI, and J. ROOZBEH, “EVALUATION OF THE GENETIC VARIATION OF NON CODING CONTROL REGION OF BK VIRUS USING NESTED-PCR SEQUENCING METHOD IN RENAL GRAFT PATIENTS,” ARMAGHAN DANESH, vol. 20, no. 2 (97), pp. 89–102, 2015, [Online]. Available: https://sid.ir/paper/78097/en

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