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Information Journal Paper

Title

In Vitro Effect of Some Medicinal Plants on Leishmania major Strain MRHO/IR/75/ER

Pages

  46-52

Abstract

 Background and objectives: Leishmaniasis is a tropical disease caused by protozoan parasites from the genus Leishmania. In this study, we aimed at investigating the in vitro anti-Leishmanial effect of essential oils of Rosmarinus officinalis, Mentha pulegium, Foeniculum vulgare, Lippia citriodora and Pelargonium graveolens. Methods: The essential oils were prepared from freshly dried and powdered plants with steam-distilled water. Iranian strain of Leishmania promastigotes was cultured in RPMI medium and the inhibitory effects of different concentrations (25, 32, 62. 5, 125, 250, 500 and 1000 μ g/ml) of the essential oils were investigated at 24, 48 and 72 hours. The number of live parasites before and after treatment with the essential oils was counted by trypan blue 10% staining and using neobar lam. Results: The essential oils significantly decreased the number of promastigotes in a dose-dependent manner (P<0. 05). However, the inhibitory effects of F. vulgare and R. officinalis essential oils were more profound compared to other essential oils. Moreover, concentrations of 500 and 1000 μ g/ml of these two essential oils exerted equal and more antiLeishmanial potency compared to glucantime, the first-line drug used for treatment of Leishmaniasis. Conclusion: Based on the results, it is recommended to evaluate the in vivo anti-Leishmanial effects of the tested essential oils, particularly F. vulgare and R. officinalis.

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    APA: Copy

    RAEISI, MOJTABA, MIRKARIMI, KAMAL, JANNAT, BEHROOZ, RAHIMI ESBOEI, BAHMAN, PAGHEH, ABDOL SATTAR, MEHRBAKHSH, ZAHRA, GHAFFARIFAR, FATEMEH, JORJANI, OGHLNIAZ, & FOROUTAN, MASOUD. (2020). In Vitro Effect of Some Medicinal Plants on Leishmania major Strain MRHO/IR/75/ER. MEDICAL LABORATORY JOURNAL, 14(4), 46-52. SID. https://sid.ir/paper/782145/en

    Vancouver: Copy

    RAEISI MOJTABA, MIRKARIMI KAMAL, JANNAT BEHROOZ, RAHIMI ESBOEI BAHMAN, PAGHEH ABDOL SATTAR, MEHRBAKHSH ZAHRA, GHAFFARIFAR FATEMEH, JORJANI OGHLNIAZ, FOROUTAN MASOUD. In Vitro Effect of Some Medicinal Plants on Leishmania major Strain MRHO/IR/75/ER. MEDICAL LABORATORY JOURNAL[Internet]. 2020;14(4):46-52. Available from: https://sid.ir/paper/782145/en

    IEEE: Copy

    MOJTABA RAEISI, KAMAL MIRKARIMI, BEHROOZ JANNAT, BAHMAN RAHIMI ESBOEI, ABDOL SATTAR PAGHEH, ZAHRA MEHRBAKHSH, FATEMEH GHAFFARIFAR, OGHLNIAZ JORJANI, and MASOUD FOROUTAN, “In Vitro Effect of Some Medicinal Plants on Leishmania major Strain MRHO/IR/75/ER,” MEDICAL LABORATORY JOURNAL, vol. 14, no. 4, pp. 46–52, 2020, [Online]. Available: https://sid.ir/paper/782145/en

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