THE EFFECT OF EXTRACT FROM AERIAL PARTS OFJUNIPERUS EXCELSA PLANT ON PROLIFERATION AND APOPTOSIS OF ACUTE LYMPHOBLASTIC LEUKEMIA CELL LINES, NALM-6 AND REH

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DARVISHI M.; ESMAEILI S.; DEHGHAN NAYERI N.; MASHATI P.; GHAREHBAGHIAN A.

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Journal: THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON) Year:2017 | Volume:13 | Issue:4 Page(s): 304-313

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Title

THE EFFECT OF EXTRACT FROM AERIAL PARTS OFJUNIPERUS EXCELSA PLANT ON PROLIFERATION AND APOPTOSIS OF ACUTE LYMPHOBLASTIC LEUKEMIA CELL LINES, NALM-6 AND REH

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Keywords

ACUTE LYMPHOBLASTIC LEUKEMIAQ2

JUNIPERUS EXCELSAQ2

CYTOTOXICITYQ2

APOPTOSISQ2

Abstract

Background and Objectives ACUTE LYMPHOBLASTIC LEUKEMIA (ALL) is the most common childhood malignancy. The side effects of chemotherapeutic drugs have primarily lead to the increased use of natural products for cancer treatment. Juniperus excels a, a medicinal herb, was reported to show antiproliferative effects on various cancer cells. In this study, cytotoxic and apoptotic effects of extract from aerial parts of Juniperus excels a plant were investigated on ACUTE LYMPHOBLASTIC LEUKEMIA cell lines, Nalm-6 and Reh.Materials and Methods In this basic research, Nalm-6 and Reh were cultured and then they were treated with various concentrations of J. excels a extract for 48 and 72 h and then the cell viability was evaluated by using MTT assay. APOPTOSIS also was assessed by caspase 3 activity assay and flow cytometry following Annexin V and Propidium iodide staining. Statistical analysis was assessed by one way ANOVA test and SPSS 23.Results MTT assay results show that J. excels a extract concentrations of 3, 4 and 5 mg/ml significantly reduce percentage of alive cells (p<0.001). Flow cytometry results also show that J. excels a extract significantly increase percentage of apoptotic cells compared with control groups (p< 0.001). Caspase 3 activity assay results show that caspase 3 activity was significantly increased in treated cells (p<0.05).Conclusions Our study shows that extract from aerial parts of J. excels a has cytotoxic and apoptotic effects on Nalm-6 and Reh cells.

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References

CYTOTOXIC ACTIVITY SCREENING OF SOME MEDICINAL PLANTS FROM SOUTH OF IRAN

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APA: Copy

DARVISHI, M., ESMAEILI, S., DEHGHAN NAYERI, N., MASHATI, P., & GHAREHBAGHIAN, A.. (2017). THE EFFECT OF EXTRACT FROM AERIAL PARTS OFJUNIPERUS EXCELSA PLANT ON PROLIFERATION AND APOPTOSIS OF ACUTE LYMPHOBLASTIC LEUKEMIA CELL LINES, NALM-6 AND REH. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 13(4), 304-313. SID. https://sid.ir/paper/78369/en

Vancouver: Copy

DARVISHI M., ESMAEILI S., DEHGHAN NAYERI N., MASHATI P., GHAREHBAGHIAN A.. THE EFFECT OF EXTRACT FROM AERIAL PARTS OFJUNIPERUS EXCELSA PLANT ON PROLIFERATION AND APOPTOSIS OF ACUTE LYMPHOBLASTIC LEUKEMIA CELL LINES, NALM-6 AND REH. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2017;13(4):304-313. Available from: https://sid.ir/paper/78369/en

IEEE: Copy

M. DARVISHI, S. ESMAEILI, N. DEHGHAN NAYERI, P. MASHATI, and A. GHAREHBAGHIAN, “THE EFFECT OF EXTRACT FROM AERIAL PARTS OFJUNIPERUS EXCELSA PLANT ON PROLIFERATION AND APOPTOSIS OF ACUTE LYMPHOBLASTIC LEUKEMIA CELL LINES, NALM-6 AND REH,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 13, no. 4, pp. 304–313, 2017, [Online]. Available: https://sid.ir/paper/78369/en

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