THE EFFECT OF METHANOLIC EXTRACT OF AERIAL PARTS OF ARTEMISIA ANNUA ON PROLIFERATION AND APOPTOSIS OF ACUTE LYMPHOBLASTIC LEUKEMIA CELL LINES, NALM-6 AND REH

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MASHATI P.; ESMAEILI S.; DEHGHAN NAYERI N.; DARVISHI M.; GHAREHBAGHIAN A.

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Journal: THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON) Year:2017 | Volume:14 | Issue:1 Page(s): 34-42

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Title

THE EFFECT OF METHANOLIC EXTRACT OF AERIAL PARTS OF ARTEMISIA ANNUA ON PROLIFERATION AND APOPTOSIS OF ACUTE LYMPHOBLASTIC LEUKEMIA CELL LINES, NALM-6 AND REH

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ACUTE LYMPHOID LEUKEMIAQ2

ARTEMISIA ANNUAQ1

APOPTOSISQ2

Abstract

Background and Objectives: Acute lymphoblastic leukemia (ALL) is the most common malignancy among children. The use of natural compounds has invoked a lot of interest in treatment of malignancies due to the adverse effects of chemotherapy treatment. ARTEMISIA ANNUA was reported to show cytotoxic effects on various cancer cell lines. In this study, cytotoxic effects of ARTEMISIA ANNUA extract were investigated on acute lymphoblastic leukemia cell lines.Materials and Methods: In this basic research, Nalm-6 and Reh cells were cultured and then treated with various concentrations of ARTEMISIA ANNUA extract; afterwards, the cell viability was evaluated using MTT assay for 48 and 72 h. The Caspase 3 activity assay and flow cytometry following Annexin V and Propidium iodide staining were used to assess APOPTOSIS. Statistical analysis was evaluated by one-way ANOVA test.Results: A. annua extract showed IC50 of 90 mg/ml on Nalm-6 and IC50 of 70 μg/ml on Reh cells after 48 h; the cytotoxic effect after 72 h was noticeable (p<0.001). Flow cytometry results also show that A. annua extract concentration of 40 mg/ml increases the percentage of apoptotic cells compared with control groups (p<0.05). Significant increase in Caspase 3 activity was observed after treatment with ARTEMISIA ANNUA compared with control groups (p<0.01).Conclusions: Our results show that methanolic extract of aerial parts of A. annua exerting cytotoxic and inhibitory effects on Nalm-6 and Reh cells.

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APA: Copy

MASHATI, P., ESMAEILI, S., DEHGHAN NAYERI, N., DARVISHI, M., & GHAREHBAGHIAN, A.. (2017). THE EFFECT OF METHANOLIC EXTRACT OF AERIAL PARTS OF ARTEMISIA ANNUA ON PROLIFERATION AND APOPTOSIS OF ACUTE LYMPHOBLASTIC LEUKEMIA CELL LINES, NALM-6 AND REH. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 14(1), 34-42. SID. https://sid.ir/paper/78516/en

Vancouver: Copy

MASHATI P., ESMAEILI S., DEHGHAN NAYERI N., DARVISHI M., GHAREHBAGHIAN A.. THE EFFECT OF METHANOLIC EXTRACT OF AERIAL PARTS OF ARTEMISIA ANNUA ON PROLIFERATION AND APOPTOSIS OF ACUTE LYMPHOBLASTIC LEUKEMIA CELL LINES, NALM-6 AND REH. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2017;14(1):34-42. Available from: https://sid.ir/paper/78516/en

IEEE: Copy

P. MASHATI, S. ESMAEILI, N. DEHGHAN NAYERI, M. DARVISHI, and A. GHAREHBAGHIAN, “THE EFFECT OF METHANOLIC EXTRACT OF AERIAL PARTS OF ARTEMISIA ANNUA ON PROLIFERATION AND APOPTOSIS OF ACUTE LYMPHOBLASTIC LEUKEMIA CELL LINES, NALM-6 AND REH,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 14, no. 1, pp. 34–42, 2017, [Online]. Available: https://sid.ir/paper/78516/en

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