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Title

The Role of Helicobacter pylori Proinflammatory Outer Membrane Protein and Propolis in Immunomodulation on U937 Macrophage Cell Model

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Abstract

 Background: Regarding the important role of proinflammatory outer membrane protein (OipA) in the pathogenesis of Helicobacter pylori infection and immunomodulatory activity of Propolis, we aimed to evaluate the immunogenicity effect of a purified recombinant OipA protein and Propolis in the induction of two cytokines, interferon-gamma (IFN-γ; ) and interleukin-4 (IL-4), in a macrophage cell model. Materials and Methods: The recombinant protein used in the present study corresponding to the OipA expressing a 34-35 kDa protein. OipA protein was purified by Ni-NTA affinity chromatography. The purified OipA protein (2. 5-40 μ g /mL) and the Propolis ethanolic extract (5-40 μ g/mL) were incubated with phorbol 12-myristate 13-acetate-treated human myelomonocytic cell line U937 cells. IL-4 and IFN-γ; levels were measured after 48 hours of incubation using enzyme-linked immunosorbent assay. Results: The amounts of IL-4 and IFN-γ; were significantly increased. The optimum concentration of OipA for the secretion of IL-4 was 5 μ g/ml (P<0. 0001). At higher concentrations, the amount of IL-4 diminished until suppression at 40 μ g/mL. The optimum concentration of Propolis, resulting in the most significant increased secretion of both IL-4 and IFN-γ; was 40 μ g/mL (P=0. 0001 and P=0. 0004). Conclusion: We found that an OipA concentration of 10 μ g/mL was more effective for IFN-γ; production; however, it was not effective for the high production of IL-4. Therefore, it is postulated that the OipA could mainly induce a Th1 response through the production of IFN-γ; . We also observed Propolis’ s capability to induce IFN-γ; production; however, the effective concentration for this was the same as for IL-4. Therefore, as an adjuvant, proper concentration of Propolis is required for OipA to give the optimum response.

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    APA: Copy

    Soudi, Hengameh, FALSAFI, TAHEREH, GHARAVI, SARA, & Mahboubi, Mohaddeseh. (2020). The Role of Helicobacter pylori Proinflammatory Outer Membrane Protein and Propolis in Immunomodulation on U937 Macrophage Cell Model. GALEN MEDICAL JOURNAL, 9(1), 0-0. SID. https://sid.ir/paper/783774/en

    Vancouver: Copy

    Soudi Hengameh, FALSAFI TAHEREH, GHARAVI SARA, Mahboubi Mohaddeseh. The Role of Helicobacter pylori Proinflammatory Outer Membrane Protein and Propolis in Immunomodulation on U937 Macrophage Cell Model. GALEN MEDICAL JOURNAL[Internet]. 2020;9(1):0-0. Available from: https://sid.ir/paper/783774/en

    IEEE: Copy

    Hengameh Soudi, TAHEREH FALSAFI, SARA GHARAVI, and Mohaddeseh Mahboubi, “The Role of Helicobacter pylori Proinflammatory Outer Membrane Protein and Propolis in Immunomodulation on U937 Macrophage Cell Model,” GALEN MEDICAL JOURNAL, vol. 9, no. 1, pp. 0–0, 2020, [Online]. Available: https://sid.ir/paper/783774/en

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