EVALUATION OF COTRANSPLANTATION OF HUMAN MESENCHYMAL STEM CELLS AND 
UMBILICAL CORD BLOOD CD34+ CELLS WITH CFU-S ASSAY

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DELALAT B.; POURFATH ELAH A.A.; MOVASSAGHPOUR A.A.; SOLEYMANI M.; KAVIANI SAEID; RASI GHAEMI S.

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Journal: The Scientific Journal of Iranian Blood Transfusion Year:2007 | Volume:3 | Issue:4 Page(s): 343-353

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Title

EVALUATION OF COTRANSPLANTATION OF HUMAN MESENCHYMAL STEM CELLS AND UMBILICAL CORD BLOOD CD34+ CELLS WITH CFU-S ASSAY

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Keywords

STEM CELLQ3

COTRANSPLANTATIONQ4

UMBILICAL CORD BLOODQ2

MESENCHYMAL STEM CELLQ4

Abstract

Background and Objectives: UMBILICAL CORD BLOOD (UCB) contains a high number of primitive progenitor cells for transplantation. However, the rate of UCB CD34+ STEM CELL engraftment is low. In this study we examined the effect of human MSCs (mesenchymal STEM CELL) on engraftment of human UCB-derived CD34+ cells in irradiated Balb/c mice.Materials and Methods: Human UCB CD34+ cells were obtained from full-term normal deliveries by immunomagnetic techniques and MSCs were isolated by a standard methodology from bone marrow aspirates. Isolated MSCs were evaluated for cell-surface antigens of CD 166 and CD 105 by flowcytometry. The absolute count of CD34+ UCB STEM CELLs was also determined by flowcytometry. Irradiated (7Gy) Balb/c mice were transplanted intravenously with 0.2 to 1.0×106 human UCB CD34+ cells in the presence or absence of 0.25 to 1×106 human bone marrow-derived MSCs. The mice in every group on day 11 after transplantation were killed and their spleen dissected. In every group, colony assay and H and E staining were performed. To make sure of the presence of human STEM CELLs in spleen colonies, UCB CD34+ cells were labeled with super paramagnetic iron oxide (SPIO) before transplantation. The colonies in spleen then underwent Prussian blue staining. In the statistical analyis, Kruskal-Wallis test using SPSS software was employed to determine the number of colonies.Results; Flowcytometry assay showed that after purification, 90% of the cells were CD34+ and 96% marker positive for MSCs. Viablity of cells was 100%. COTRANSPLANTATION of low doses of UCB CD34+ cells (0.2 and 0.3×106) and MSC (0.5 and 1×106) resulted in a significant increase of the number of colony forming units spleen, in comparison with the engraftment of UCB CD34+ STEM CELLs without MSC after 11 days (p<0.01). Prussian blue staining showed the Fe+2 granules in UCB STEM CELLs. This indicated that cells in the colonies were engrafted UCB CD34+ STEM CELLs.Conclusions: The results showed that COTRANSPLANTATION of MSC with UCB CD34+ cells promote engraftment of UCB CD34+ cells.

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APA: Copy

DELALAT, B., POURFATH ELAH, A.A., MOVASSAGHPOUR, A.A., SOLEYMANI, M., KAVIANI, SAEID, & RASI GHAEMI, S.. (2007). EVALUATION OF COTRANSPLANTATION OF HUMAN MESENCHYMAL STEM CELLS AND UMBILICAL CORD BLOOD CD34⁺ CELLS WITH CFU-S ASSAY. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 3(4), 343-353. SID. https://sid.ir/paper/78405/en

Vancouver: Copy

DELALAT B., POURFATH ELAH A.A., MOVASSAGHPOUR A.A., SOLEYMANI M., KAVIANI SAEID, RASI GHAEMI S.. EVALUATION OF COTRANSPLANTATION OF HUMAN MESENCHYMAL STEM CELLS AND UMBILICAL CORD BLOOD CD34⁺ CELLS WITH CFU-S ASSAY. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2007;3(4):343-353. Available from: https://sid.ir/paper/78405/en

IEEE: Copy

B. DELALAT, A.A. POURFATH ELAH, A.A. MOVASSAGHPOUR, M. SOLEYMANI, SAEID KAVIANI, and S. RASI GHAEMI, “EVALUATION OF COTRANSPLANTATION OF HUMAN MESENCHYMAL STEM CELLS AND UMBILICAL CORD BLOOD CD34⁺ CELLS WITH CFU-S ASSAY,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 3, no. 4, pp. 343–353, 2007, [Online]. Available: https://sid.ir/paper/78405/en

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