IN VITRO DIFFERENTIATION OF HUMAN UMBILICAL VEIN MESENCHYMAL STEM CELLS INTO HEPATOCYTE-LIKE CELLS

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RAUFI A.; AMINI A.; AZADBAKHT M.; NIKKHOO B.; ABOOZARI M.; FATHI F.

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Journal: THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON) Year:2011 | Volume:8 | Issue:2 (31) Page(s): 79-87

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Title

IN VITRO DIFFERENTIATION OF HUMAN UMBILICAL VEIN MESENCHYMAL STEM CELLS INTO HEPATOCYTE-LIKE CELLS

Author(s)

Keywords

UMBILICAL VEINSQ3

MESENCHYMAL STEM CELLSQ2

HEPATOCYTE GROWTH FACTORQ3

ONCOSTATIN MQ3

Abstract

Background and Objectives: Umbilical vein MESENCHYMAL STEM CELLS (UVMSC) which are multipotent precursors have been recently isolated. They are capable of differentiating into various cell types including neural and cardiac cells. In this study, the differentiation ability of human UVMSC into hepatocyte cells has investigated with concentration on hepatic genes expression profile.Materials and Methods: The UVMSC were isolated and cultured in differentiation medium containing HEPATOCYTE GROWTH FACTOR (HGF) and ONCOSTATIN M (OSM) using two step protocol. Differentiation of UVMSCs into cells expressing liver-specific genes was investigated by RT-PCR and immunocytochemistry.Results: The cells showed the remarkable transition from bipolar fibroblast-like morphology to epithelial and polygonal shapes. The temporal gene expression pattern of hepatocyte-specific genes such as CK8, CK18, HNF3b, c-Met, AFP, TTR, G6P, Transthyretin, and ALB were detected during differentiation. The immunoflourescent analysis also showed that the differentiated cells were stained positively for CK-18 protein.Conclusions: Findings indicate that UVMSC has a potential for differentiation into the hepatic like cells.

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APA: Copy

RAUFI, A., AMINI, A., AZADBAKHT, M., NIKKHOO, B., ABOOZARI, M., & FATHI, F.. (2011). IN VITRO DIFFERENTIATION OF HUMAN UMBILICAL VEIN MESENCHYMAL STEM CELLS INTO HEPATOCYTE-LIKE CELLS. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 8(2 (31)), 79-87. SID. https://sid.ir/paper/78504/en

Vancouver: Copy

RAUFI A., AMINI A., AZADBAKHT M., NIKKHOO B., ABOOZARI M., FATHI F.. IN VITRO DIFFERENTIATION OF HUMAN UMBILICAL VEIN MESENCHYMAL STEM CELLS INTO HEPATOCYTE-LIKE CELLS. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2011;8(2 (31)):79-87. Available from: https://sid.ir/paper/78504/en

IEEE: Copy

A. RAUFI, A. AMINI, M. AZADBAKHT, B. NIKKHOO, M. ABOOZARI, and F. FATHI, “IN VITRO DIFFERENTIATION OF HUMAN UMBILICAL VEIN MESENCHYMAL STEM CELLS INTO HEPATOCYTE-LIKE CELLS,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 8, no. 2 (31), pp. 79–87, 2011, [Online]. Available: https://sid.ir/paper/78504/en

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