DEVELOPMENT OF NASBA-ELISA TECHNIQUE FOR DETECTION OF HIV-1 RNA

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SHAHRABI M.; FOROUZANDEH M.; SABAHI F.; PARYAN M.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

1,039

Download:

Cites:

Information Journal Paper

Title

DEVELOPMENT OF NASBA-ELISA TECHNIQUE FOR DETECTION OF HIV-1 RNA

Author(s)

SHAHRABI M. | FOROUZANDEH M. | SABAHI F. | PARYAN M. | Issue Writer Certificate

Keywords

NASBAQ3

ELISAQ3

IMMUNOASSAYQ3

HIV-1Q3

Abstract

Background and Objectives: Viral RNA is the first blood marker which appears in HIV-1 infection. RT-PCR and NASBA are the commonly used techniques for amplification of RNA. NASBA technique provides more advantages over RT-PCR. In this study, an NASBA assay combined with an easy, sensitive enzyme-linked immunosorbent assay (ELISA) was developed for detection of HIV-1 RNA.Materials and Methods: 11-dig-UTP was added to an NASBA reaction mixture and the RNA amplicons were labeled. Then, the dig-labeled NASBA products hybridized to a biotinylated specific probe in hybridization solution buffer and the hybrids were transferred to a streptoavidin-coated plate. After several washing processes and emission of nonspecific products, the final detection of the captured RNA was done by addition of anti-dig antibody-enzyme conjugate and the substrate.Results: The results demonstrated that, NASBA is an efficient method for amplification of the target genome. Detection of NASBA products by agarose gel electrophoresis showed a unique 176 bp band corresponding to the specific target. For the detection of NASBA products, an ELISA assay was performed; using 0.01 PM probe, the OD of 1.049 ± 0.11 was obtained. Conclusions: In this study, by using suitable primers and probe a highly sensitive and specific NASBAELISA method for detection of HIV-1 developed.

Cites

No record.

References

No record.

Cite

APA: Copy

SHAHRABI, M., FOROUZANDEH, M., SABAHI, F., & PARYAN, M.. (2011). DEVELOPMENT OF NASBA-ELISA TECHNIQUE FOR DETECTION OF HIV-1 RNA. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 8(1 (30)), 42-51. SID. https://sid.ir/paper/78418/en

Vancouver: Copy

SHAHRABI M., FOROUZANDEH M., SABAHI F., PARYAN M.. DEVELOPMENT OF NASBA-ELISA TECHNIQUE FOR DETECTION OF HIV-1 RNA. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2011;8(1 (30)):42-51. Available from: https://sid.ir/paper/78418/en

IEEE: Copy

M. SHAHRABI, M. FOROUZANDEH, F. SABAHI, and M. PARYAN, “DEVELOPMENT OF NASBA-ELISA TECHNIQUE FOR DETECTION OF HIV-1 RNA,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 8, no. 1 (30), pp. 42–51, 2011, [Online]. Available: https://sid.ir/paper/78418/en

Related Journal Papers