CCR5 (HIV-1 CORECEPTOR) GENE AMPLIFICTION AND DELTA L32BP MUTATION DETECTION BY PCR-ELISA

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

KHODASHENAS LIMONY S.A.; FOROUZANDEH MOGHADAM M.; MOUSAVI M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Pathobiology Research Year:2002 | Volume:5 | Issue:1 Page(s): 41-45

Download Full-Text

Persian Verion

View:

3,399

Download:

Cites:

Information Journal Paper

Title

CCR5 (HIV-1 CORECEPTOR) GENE AMPLIFICTION AND DELTA L32BP MUTATION DETECTION BY PCR-ELISA

Author(s)

KHODASHENAS LIMONY S.A. | FOROUZANDEH MOGHADAM M. | MOUSAVI M. | Issue Writer Certificate

Keywords

HIV-1Q3

CCR5Q4

PCRQ2

PCR-ELISAQ2

Abstract

Purpose: HIV -1 enters into target cell by the use of chemokine receptor CC-CKR-5. The mutant gene of this coreceptor (CCR5Δ32bp) codes a truncated protein which does not express on the cell surface play its' coreceptor function. According to the recent studies, homozygote individuals with mutant gene are immune to HIV-IR5 and hetrozygote individuals show a kind of slow development of AIDS. Due to its important function the mutation frequency of this gene has been studied in different human population.Materials and Methods: To study the existence of this mutation the PCR-ELISA technique was used for its sensitivity, specificity and speed.Results and Discussion: Genomic DNA was extracted from different blood samples. By PCR and specfic primers, a735bp fragment from CCR5 gene was amplifed, and the mutation was detected by two labeled probes (CCR-W to wild allele, CCR-M to mutant allele).CCR-W is complementary to wild allele including 32bp mutation site. The second probe (CCR-M) is complementary to the deleted region of mutant allele. For PCR-ELISA method, PCR product was labeled by digoxigenin and the 3' end of CCR Wand CCR-M was eabeled biotin-dUTP. After hybridization of the denatured PCR product with its complementary probe, the hybrids were captured in streptoavidine coated microtiter plate. Detection was performed by using anti-digoxiginin conjugated to HRP. The results were analyzed and the sensitivity of this method found to be (PCR-ELISA)<300 pg of human genomic DNA.

Cites

No record.

References

No record.

Cite

APA: Copy

KHODASHENAS LIMONY, S.A., FOROUZANDEH MOGHADAM, M., & MOUSAVI, M.. (2002). CCR5 (HIV-1 CORECEPTOR) GENE AMPLIFICTION AND DELTA L32BP MUTATION DETECTION BY PCR-ELISA. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 5(1), 41-45. SID. https://sid.ir/paper/81189/en

Vancouver: Copy

KHODASHENAS LIMONY S.A., FOROUZANDEH MOGHADAM M., MOUSAVI M.. CCR5 (HIV-1 CORECEPTOR) GENE AMPLIFICTION AND DELTA L32BP MUTATION DETECTION BY PCR-ELISA. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2002;5(1):41-45. Available from: https://sid.ir/paper/81189/en

IEEE: Copy

S.A. KHODASHENAS LIMONY, M. FOROUZANDEH MOGHADAM, and M. MOUSAVI, “CCR5 (HIV-1 CORECEPTOR) GENE AMPLIFICTION AND DELTA L32BP MUTATION DETECTION BY PCR-ELISA,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 5, no. 1, pp. 41–45, 2002, [Online]. Available: https://sid.ir/paper/81189/en

Related Journal Papers