ASSESSMENT OF PRODUCTION METHODS OF ANTIBODIES AGAINST SHEEP RED BLOOD CELLS FOR APPLICATION IN COMPLEMENT SYSTEM

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

YARI F.; HAMIDPOUR ZARE L.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON) Year:2006 | Volume:3 | Issue:1 Page(s): 45-51

Download Full-Text

Persian Verion

View:

1,742

Download:

Cites:

Information Journal Paper

Title

ASSESSMENT OF PRODUCTION METHODS OF ANTIBODIES AGAINST SHEEP RED BLOOD CELLS FOR APPLICATION IN COMPLEMENT SYSTEM

Author(s)

YARI F. | HAMIDPOUR ZARE L. | Issue Writer Certificate

Keywords

FORSSMAN ANTIGENQ4

CH50Q4

SRBCQ4

Abstract

Background and ObjectivesCH50 method is used for the evaluation of complement system. In this method, sheep red blood cells (SRBCs) are sensitized with specific antibodies. Then, the capability of the complement system in the completion of the hemolytic reaction is assessed. In this study because of need for anti-SRBC antibody in CH50 test, it was produced in rabbit. This antibody is called "Amboceptor".Materials and Methods In order to perform this study, SRBCs or their membrane antigens were injected into rabbits with different methods. Produced antibodies were analyzed in CH50 method using human pooled sera (derived from at least 15 blood donors). Finally the optical densities (ODs) related to the lysis of SRBCs were compared. It is obvious that the amount of OD is correlated with the efficiency of the antibody.ResultsIntravenous or intraperitoneal injections of SRBCs repeatedly result in the formation of high titer antibodies, but the rapid death of the animals due to the anaphylactic shock occurs. While the usage of sonicated or heat-treated antigens of SRBCs in the immunization leads to the formation of antibodies with high specificity and titer; it does not threaten the life of the animals.ConclusionsProduction of anti-SRBC antibodies is applicable using different methods. This study shows that the use of the membrane or heat-stable antigens of SRBCs, which are prepared using sonication and heating (103ºC) respectively, has some advantages. These antigens are safe for rabbits and the titer of the antibody is high enough to be used in CH50 test.

Cites

No record.

References

No record.

Cite

APA: Copy

YARI, F., & HAMIDPOUR ZARE, L.. (2006). ASSESSMENT OF PRODUCTION METHODS OF ANTIBODIES AGAINST SHEEP RED BLOOD CELLS FOR APPLICATION IN COMPLEMENT SYSTEM. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 3(1), 45-51. SID. https://sid.ir/paper/78449/en

Vancouver: Copy

YARI F., HAMIDPOUR ZARE L.. ASSESSMENT OF PRODUCTION METHODS OF ANTIBODIES AGAINST SHEEP RED BLOOD CELLS FOR APPLICATION IN COMPLEMENT SYSTEM. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2006;3(1):45-51. Available from: https://sid.ir/paper/78449/en

IEEE: Copy

F. YARI, and L. HAMIDPOUR ZARE, “ASSESSMENT OF PRODUCTION METHODS OF ANTIBODIES AGAINST SHEEP RED BLOOD CELLS FOR APPLICATION IN COMPLEMENT SYSTEM,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 3, no. 1, pp. 45–51, 2006, [Online]. Available: https://sid.ir/paper/78449/en

Related Journal Papers