THE EFFECT OF ARSENIC TRIOXIDE TREATMENT ON MITOCHONDRIAL APOPTOTIC GENE EXPRESSION IN ACUTE PROMYELOCYTIC LEUKEMIA CELL LINE

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SHARIFIZADEH MAEDEH; HASHEMI MEHRDAD; MOUMENI MAJID; ALI MOGHADAM K.; GHAVAMZADEH ARDESHIR; GHAFARI H.A.

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Journal: THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON) Year:2010 | Volume:7 | Issue:3 (28) Page(s): 138-147

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Title

THE EFFECT OF ARSENIC TRIOXIDE TREATMENT ON MITOCHONDRIAL APOPTOTIC GENE EXPRESSION IN ACUTE PROMYELOCYTIC LEUKEMIA CELL LINE

Author(s)

Keywords

PROMYELOCYTIC LEUKEMIAQ3

ACUTEQ3

ARSENIC TRIOXIDEQ2

APOPTOSISQ3

CASPASE 3Q3

BCL-2 GENESQ3

Abstract

Background and Objectives: ACUTE PROMYELOCYTIC LEUKEMIA (APL) is one of the most malignant forms of ACUTE leukemia with a fatal course of only weeks which represents 10-15% of AML in adults. ARSENIC TRIOXIDE as a single agent factor (without chemotherapy) is the treatment of choice for APL patients; it induces cell death through APOPTOSIS but the mechanism by which arsenic targets APOPTOSIS and dramatically affects gene expression remains poorly understood. Since arsenic is used as first line treatment in Iran, it is worth investigating its effect on expression of genes involved in APL.Materials and Methods: In this descriptive study, to understand the underlying mechanisms of cell death induction by arsenic, we treated NB4 cell line in a dose and time dependent manner. Extracting RNA and synthesis of cDNA, gene expression of apoptotic genes in mitochondrial pathway including caspase3, Mcl-1 and Bcl-2 was analyzed through Real-Time PCR.Results: Our findings showed that As2O3-induced cell death was paralleled by reduced expression of the antiapoptotic protein Bcl-2 but the expression of Caspase3 and Mcl-1 did not change after arsenic treatment.Conclusions: These results suggest that changes in Bcl-2 gene expression may be one of the mechanisms of action of arsenic in induction of APOPTOSIS, while Caspase3 and Mcl-1 gene expression are not affected by arsenic at the transcriptional level.

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APA: Copy

SHARIFIZADEH, MAEDEH, HASHEMI, MEHRDAD, MOUMENI, MAJID, ALI MOGHADAM, K., GHAVAMZADEH, ARDESHIR, & GHAFARI, H.A.. (2010). THE EFFECT OF ARSENIC TRIOXIDE TREATMENT ON MITOCHONDRIAL APOPTOTIC GENE EXPRESSION IN ACUTE PROMYELOCYTIC LEUKEMIA CELL LINE. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 7(3 (28)), 138-147. SID. https://sid.ir/paper/78466/en

Vancouver: Copy

SHARIFIZADEH MAEDEH, HASHEMI MEHRDAD, MOUMENI MAJID, ALI MOGHADAM K., GHAVAMZADEH ARDESHIR, GHAFARI H.A.. THE EFFECT OF ARSENIC TRIOXIDE TREATMENT ON MITOCHONDRIAL APOPTOTIC GENE EXPRESSION IN ACUTE PROMYELOCYTIC LEUKEMIA CELL LINE. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2010;7(3 (28)):138-147. Available from: https://sid.ir/paper/78466/en

IEEE: Copy

MAEDEH SHARIFIZADEH, MEHRDAD HASHEMI, MAJID MOUMENI, K. ALI MOGHADAM, ARDESHIR GHAVAMZADEH, and H.A. GHAFARI, “THE EFFECT OF ARSENIC TRIOXIDE TREATMENT ON MITOCHONDRIAL APOPTOTIC GENE EXPRESSION IN ACUTE PROMYELOCYTIC LEUKEMIA CELL LINE,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 7, no. 3 (28), pp. 138–147, 2010, [Online]. Available: https://sid.ir/paper/78466/en

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