MOLECULAR DIAGNOSIS OF B CELL NON-HODGKIN LYMPHOMA BY EVALUATION OF IMMUNOGLOBULIN HEAVY CHAIN GENE REARRANGEMENT

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YOUSEFIAN A.A.F.; POUPAK B.; ABOU ALGHASEMI HASAN; AZAR KEYVAN A.; FARHADI LANGEROUDI MOHAMMAD; SADEGHIPOUR ALI REZA; JEYHOUNIAN MOZHGAN; JAVADPOUR KHAYAT S.M.; ZARE KHANDAN; FARAAHANI KOBRA; SALAHMAND MITRA

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Journal: THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON) Year:2008 | Volume:5 | Issue:3 (20) Page(s): 157-166

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Title

MOLECULAR DIAGNOSIS OF B CELL NON-HODGKIN LYMPHOMA BY EVALUATION OF IMMUNOGLOBULIN HEAVY CHAIN GENE REARRANGEMENT

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Keywords

GENE REARRANGEMENTQ4

NON-HODGKIN LYMPHOMAQ4

IMMUNOGLOBULINQ3

Abstract

Background and Objectives: Rearrangement of V, D, and J segments of IMMUNOGLOBULIN heavy chain gene with inserted or deleted nucleotides within rearranged segments makes unique hypervariable regions (CDR-3). These regions can be used for evaluation of B cell clonality for the purpose of molecular diagnosis of NON-HODGKIN LYMPHOMA (NHL) and for confirmatory diagnosis in suspicious cases.Materials and Methods: In this study, samples of 42 patients were collected from Taleghani, Baqhiyatalah, and Aliasghar hospitals; out of this number, there were 22 patients with diagnosis of B cell NHL, 10 with reactive hyperplasia, and 10 with malignant lymphoma. After DNA extraction from formalin fixed paraffin embedded tissues, PCR was done using consensus primers for amplification of CDR-3 region. PCR products were analyzed after heteroduplex analysis using polyacrylamide gel electrophoresis and silver stain.Results: Clonal patterns in group 1 (B cell NHL), 2 (reactive and follicular hyperplasia), and 3 (morphological diagnosis without immunohistochemistry) were observed in 77.2%, 0%, and 70% of patients, respectively.Conclusions: Our findings are compatible with other international studies with minor differences. The diagnosis of B-cell lymphoid malignancy can frequently be substantiated by detecting clonal IMMUNOGLOBULIN heavy chain (IGH) GENE REARRANGEMENT.

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APA: Copy

YOUSEFIAN, A.A.F., POUPAK, B., ABOU ALGHASEMI, HASAN, AZAR KEYVAN, A., FARHADI LANGEROUDI, MOHAMMAD, SADEGHIPOUR, ALI REZA, JEYHOUNIAN, MOZHGAN, JAVADPOUR KHAYAT, S.M., ZARE, KHANDAN, FARAAHANI, KOBRA, & SALAHMAND, MITRA. (2008). MOLECULAR DIAGNOSIS OF B CELL NON-HODGKIN LYMPHOMA BY EVALUATION OF IMMUNOGLOBULIN HEAVY CHAIN GENE REARRANGEMENT. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 5(3 (20)), 157-166. SID. https://sid.ir/paper/78498/en

Vancouver: Copy

YOUSEFIAN A.A.F., POUPAK B., ABOU ALGHASEMI HASAN, AZAR KEYVAN A., FARHADI LANGEROUDI MOHAMMAD, SADEGHIPOUR ALI REZA, JEYHOUNIAN MOZHGAN, JAVADPOUR KHAYAT S.M., ZARE KHANDAN, FARAAHANI KOBRA, SALAHMAND MITRA. MOLECULAR DIAGNOSIS OF B CELL NON-HODGKIN LYMPHOMA BY EVALUATION OF IMMUNOGLOBULIN HEAVY CHAIN GENE REARRANGEMENT. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2008;5(3 (20)):157-166. Available from: https://sid.ir/paper/78498/en

IEEE: Copy

A.A.F. YOUSEFIAN, B. POUPAK, HASAN ABOU ALGHASEMI, A. AZAR KEYVAN, MOHAMMAD FARHADI LANGEROUDI, ALI REZA SADEGHIPOUR, MOZHGAN JEYHOUNIAN, S.M. JAVADPOUR KHAYAT, KHANDAN ZARE, KOBRA FARAAHANI, and MITRA SALAHMAND, “MOLECULAR DIAGNOSIS OF B CELL NON-HODGKIN LYMPHOMA BY EVALUATION OF IMMUNOGLOBULIN HEAVY CHAIN GENE REARRANGEMENT,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 5, no. 3 (20), pp. 157–166, 2008, [Online]. Available: https://sid.ir/paper/78498/en

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