CELLULAR AND MOLECULAR EVALUATION OF ENDOTHELIAL PROGENITOR CELLS AFTER SELECTIVE ISOLATION FROM PERIPHERAL BLOOD AND COMPARISON OF THEIR TRANSECTION BY LIPOFECTION AND ELECTROPORATION

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FATHI F.; BAGHBAN ESLAMINEZHAD M.R.; KHADEM ERFAN M.B.; YUKI ASAHARA T.

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Journal: THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON) Year:2006 | Volume:3 | Issue:2 Page(s): 121-131

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Title

CELLULAR AND MOLECULAR EVALUATION OF ENDOTHELIAL PROGENITOR CELLS AFTER SELECTIVE ISOLATION FROM PERIPHERAL BLOOD AND COMPARISON OF THEIR TRANSECTION BY LIPOFECTION AND ELECTROPORATION

Author(s)

FATHI F. | BAGHBAN ESLAMINEZHAD M.R. | KHADEM ERFAN M.B. | YUKI ASAHARA T. | Issue Writer Certificate

Keywords

ENDOTHELIAL PROGENITOR CELLS (EPCS)Q4

ISOLATIONQ4

LIPOFECTINQ4

ELECTROPORATIONQ3

Abstract

Background and Objectives: Bone marrow of adults contains a subtype of progenitor cells that has the capacity to differentiate into mature endothelial cells and has therefore been termed as endothelial progenitor cells (EPCs). Clinical studies employing EPCs for revascularization of ischemicorgans have just started to be conducted. In the present research, endothelial progenitor cells were isolated from peripheral blood and transfected by lipofection method. Materials and Methods: All mononuclear cells were isolated from peripheral blood by vivo expansion method and cultured in fibronectin coated dishes and basal EC medium. In day 7, the cells were tripsinized and examined by immunocytochemical and molecular evaluation methods; then, ELECTROPORATION and lipofection methods were used for transfection of EPCs. Results: Observations showed isolated cells were proliferated on fibronectin coated surfaces. The cells were able to incorporate Acl-Dil and express CD31, CD34, KDR, VECAM-1, Tie-1 genes, CD31, CD34, Lectin and KDR antigens. The cells were also transfected by lipofection method. Conclusions: It can be concluded that hEPCs express specific endothelial genes in day 7 and are transfected by lipofection method with low efficiency.

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APA: Copy

FATHI, F., BAGHBAN ESLAMINEZHAD, M.R., KHADEM ERFAN, M.B., & YUKI ASAHARA, T.. (2006). CELLULAR AND MOLECULAR EVALUATION OF ENDOTHELIAL PROGENITOR CELLS AFTER SELECTIVE ISOLATION FROM PERIPHERAL BLOOD AND COMPARISON OF THEIR TRANSECTION BY LIPOFECTION AND ELECTROPORATION. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 3(2), 121-131. SID. https://sid.ir/paper/78630/en

Vancouver: Copy

FATHI F., BAGHBAN ESLAMINEZHAD M.R., KHADEM ERFAN M.B., YUKI ASAHARA T.. CELLULAR AND MOLECULAR EVALUATION OF ENDOTHELIAL PROGENITOR CELLS AFTER SELECTIVE ISOLATION FROM PERIPHERAL BLOOD AND COMPARISON OF THEIR TRANSECTION BY LIPOFECTION AND ELECTROPORATION. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2006;3(2):121-131. Available from: https://sid.ir/paper/78630/en

IEEE: Copy

F. FATHI, M.R. BAGHBAN ESLAMINEZHAD, M.B. KHADEM ERFAN, and T. YUKI ASAHARA, “CELLULAR AND MOLECULAR EVALUATION OF ENDOTHELIAL PROGENITOR CELLS AFTER SELECTIVE ISOLATION FROM PERIPHERAL BLOOD AND COMPARISON OF THEIR TRANSECTION BY LIPOFECTION AND ELECTROPORATION,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 3, no. 2, pp. 121–131, 2006, [Online]. Available: https://sid.ir/paper/78630/en

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