مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

1,130
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

0
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

EVALUATION OF EFFECTS OF DICLOFENAC ON THE PROLIFERATION AND DIFFERENTIATION OF PC12 CELLS IN VITRO

Pages

  15-21

Abstract

 Background & Objective: DICLOFENAC is a non-steroidal, anti-inflamatory drug that is prescribed as an analgesic. However, there is little known about the effects of DICLOFENAC on the neural cells. In this study, we investigated the effects of DICLOFENAC as sodium salt on the proliferation and DIFFERENTIATION of PC12 cells.Materials & Methods: This expeimental study was done in Kerman neuroscience research center during 2004. The cell proliferation was evaluated by using XTT assay in the both free-serum neurobasal medium supplemented with B27 supplement and DMEM/F12 medium containing 10% FBS. The nerve growth factor (NGF) – induced DIFFERENTIATION was assessed by measuring the neurite length for each treatment.Results: The drug toxicity was exhibited at the higher concentrations of 310 mM in the supplemented neurobasal medium. The treatment of cells in the DMEM/F12 medium increased their sensitivity to DICLOFENAC, with 40 and 85% growth inhibition at the 155 and 310 mM concentrations, respectively. The different generics of drug exhibited a equal toxic effects on the PC12 cells. The NGF- induced DIFFERENTIATION was not reduced by toxic and subtoxic concentrations of DICLOFENAC.Conclusion: This study indicated that DICLOFENAC may be able to exhibit its neurotoxic effects through growth inhibition, but not DIFFERENTIATION inhibition. B27 supplement has several antioxidant compounds. Therefore, the difference of DICLOFENAC cytotoxic effects in two culture media suggest that drug CYTOTOXICITY may be related to the oxidative stress.

Cites

  • No record.
  • References

  • No record.
  • Cite

    APA: Copy

    RAJABALIAN, SAEID, MEYMANDI, M.SH., DABIRI, SH., & HOSSEIN, RAFAT. (2007). EVALUATION OF EFFECTS OF DICLOFENAC ON THE PROLIFERATION AND DIFFERENTIATION OF PC12 CELLS IN VITRO. JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES, 9(3 (23)), 15-21. SID. https://sid.ir/paper/78928/en

    Vancouver: Copy

    RAJABALIAN SAEID, MEYMANDI M.SH., DABIRI SH., HOSSEIN RAFAT. EVALUATION OF EFFECTS OF DICLOFENAC ON THE PROLIFERATION AND DIFFERENTIATION OF PC12 CELLS IN VITRO. JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2007;9(3 (23)):15-21. Available from: https://sid.ir/paper/78928/en

    IEEE: Copy

    SAEID RAJABALIAN, M.SH. MEYMANDI, SH. DABIRI, and RAFAT HOSSEIN, “EVALUATION OF EFFECTS OF DICLOFENAC ON THE PROLIFERATION AND DIFFERENTIATION OF PC12 CELLS IN VITRO,” JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES, vol. 9, no. 3 (23), pp. 15–21, 2007, [Online]. Available: https://sid.ir/paper/78928/en

    Related Journal Papers

    Related Seminar Papers

  • No record.
  • Related Plans

  • No record.
  • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button